User:Moira M. Esson/Notebook/CHEM-571/2013/09/03

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(Preparation of Inosine Stock Solutions)
(Objectives)
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# Prepare inosine solutions of various concentrations to determine the molar absorptivity of [http://en.wikipedia.org/wiki/Inosine inosine].
# Prepare inosine solutions of various concentrations to determine the molar absorptivity of [http://en.wikipedia.org/wiki/Inosine inosine].
# Prepare calibration curves for adenosine and inosine.
# Prepare calibration curves for adenosine and inosine.
 +
# Analyze the data of all of the combined CHEM-571 data.
<br>
<br>
 +
==Preparation of Adenosine Stock solution==
==Preparation of Adenosine Stock solution==
General Protocol
General Protocol

Revision as of 14:06, 3 September 2013

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Objectives

  1. Prepare adenosine solutions of various concentrations to determine the molar absorptivity of adenosine.
  2. Prepare inosine solutions of various concentrations to determine the molar absorptivity of inosine.
  3. Prepare calibration curves for adenosine and inosine.
  4. Analyze the data of all of the combined CHEM-571 data.


Preparation of Adenosine Stock solution

General Protocol

  1. In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H2O up until the white line.
    • Note: To ensure the full addition of all of the adenosine, a small amount of deionized H2O was placed on the weigh boat and a pipette was used to remove all of the H2O on the weigh boat.s
  2. Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
    • Note:The solution should be shaken vigorously for a minimum of 5 minutes.
  3. Pour the solution into a 50mL falcon tube for later storage.
  4. Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.


  • Needed Stock Solutions for Adenosine


Adenosine solution concentrations (M)
3.00x10-5
2.50x10-5
2.00x10-5
1.50x10-5
1.00x10-5
0.50x10-5
RANDOM


  • Stock Solution Preparation for Adenosine
Total Mass [g] Stock Volume [mL] Concentration [M]
0.2006500.015


  • Serial Dilution of Adenosine Stock Solution
Dilution 'M1'[mol/L] 'V1' [mL] 'M2'[mol/L] 'V2' [mL]
10.0150.11.5E-410
20.0150.253.0E-510
31.5E-41.667 2.5E-510
41.5E-41.3332.0E-510
51.5E-41.01.5E-510
61.5E-40.6671.0E-510
71.5E-40.3330.5E-510
81.5E-40.50.75E-510


Preparation of Inosine Stock Solutions

General Protocol

  1. In a clean 25mL volumetric flask, add 0.1347g inosine. Fill the volumetric flask with deionized H2O up until the white line.
    • Note: To ensure the full addition of all of the inosine, a small amount of deionized H2O was placed on the weigh boat and a pipette was used to remove all of the H2O on the weigh boat.s
  2. Cap the volumetric flask and shake the volumetric flask vigorously until the inosine is completely dissolved.
    • Note:The solution should be shaken vigorously for a minimum of 3 minutes.
  3. Pour the solution into a 50mL falcon tube for later storage.
  4. Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.


Notes

  • Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed throughly with deionized H2O.

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