User:Moira M. Esson/Notebook/CHEM-571/2013/09/03: Difference between revisions

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==Notes==
==Notes==
*Originally the adenosine stock solution was prepared  
*Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed throughly with deionized H<sub>2</sub>O.
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Revision as of 11:25, 3 September 2013

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Objectives

  1. Prepare adenosine solutions of various concentrations to determine the molar absorptivity of adenosine.
  2. Prepare inosine solutions of various concentrations to determine the molar absorptivity of inosine.
  3. Prepare calibration curves for adenosine and inosine.


Preparation of Adenosine Stock solution

  1. In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H2O up until the white line.
    • Note: To ensure the full addition of all of the adenosine, a small amount of deionized H2O was placed on the weigh boat and a pipette was used to remove all of the H2O on the weigh boat.s
  2. Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
    • Note:The solution should be shaken vigorously for a minimum of 5 minutes.
  3. Pour the solution into a 50mL falcon tube for later storage.
  4. Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.


  • Needed Stock Solutions for Adenosine
Adenosine solution concentrations (M)
3.00x10-5
2.50x10-5
2.00x10-5
1.50x10-5
1.00x10-5
0.50x10-5
RANDOM


  • Stock Solution Preparation for Adenosine
Total Mass [g] Stock Volume [mL] Concentration [M]
0.2006 50 0.015


  • Serial Dilution of Adenosine Stock Solution
Dilution 'M1'[mol/L] 'V1' [mL] 'M2'[mol/L] 'V2' [mL]
1 0.015 0.1 1.5E-4 10
2 0.015 0.25 3.0E-5 10
3 1.5E-4 1.667 2.5E-5 10
4 1.5E-4 1.333 2.0E-5 10
5 1.5E-4 1.0 1.5E-5 10
6 1.5E-4 0.667 1.0E-5 10
7 1.5E-4 0.333 0.5E-5 10
8 1.5E-4 0.5 0.75E-5 10


Notes

  • Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed throughly with deionized H2O.


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