User:Moira M. Esson/Notebook/CHEM-571/2013/09/03

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(Preparation of Adenosine stock solutions)
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# Prepare calibration curves for adenosine and inosine.
# Prepare calibration curves for adenosine and inosine.
<br>
<br>
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==Preparation of Adenosine stock solutions==
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==Preparation of Adenosine Stock solution
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#In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H<sub>2</sub>O up until the white line.
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#Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
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#*'''Note''':The solution should be shaken vigorously for a minimum of 5 minutes.
 +
#Pour the solution into a 50mL falcon tube for later storage.
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#Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H<sub>2</sub>O.
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<br>
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* Stock Solution Preparation for Adenosine 
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Total Mass [g]'''
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| align="center" style="background:#f0f0f0;"|'''Stock Volume [mL]'''
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| align="center" style="background:#f0f0f0;"|'''Concentration ['''M''']'''
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|-
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| 0.2006||50||0.015|||
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|}
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<br>
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*Serial Dilution of Adenosine Stock Solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Dilution'''
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| align="center" style="background:#f0f0f0;"|''''''M<sub>1'''[mol/L]'''
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| align="center" style="background:#f0f0f0;"|''''''V<sub>1''' [mL]'''
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| align="center" style="background:#f0f0f0;"|''''''M<sub>2'''[mol/L]'''
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| align="center" style="background:#f0f0f0;"|''''''V<sub>2''' [mL]'''
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|-
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| 1||0.015||0.1||1.5E-4||10||
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|-
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| 2||0.015||0.25||3.0E-5||10||
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|-
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| 3||1.5E-4||1.667 ||2.5E-5||10||
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|-
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| 4||1.5E-4||1.333||2.0E-5||10||
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|-
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| 5||1.5E-4||1.0||1.5E-5||10||
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|-
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| 6||1.5E-4||0.667||1.0E-5||10||
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|-
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| 7||1.5E-4||0.333||0.5E-5||10||
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|-
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| 8||1.5E-4||0.5||0.75E-5||10||
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|}
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Revision as of 14:16, 3 September 2013

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Objectives

  1. Prepare adenosine solutions of various concentrations to determine the molar absorptivity of adenosine.
  2. Prepare inosine solutions of various concentrations to determine the molar absorptivity of inosine.
  3. Prepare calibration curves for adenosine and inosine.


==Preparation of Adenosine Stock solution

  1. In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H2O up until the white line.
  2. Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
    • Note:The solution should be shaken vigorously for a minimum of 5 minutes.
  3. Pour the solution into a 50mL falcon tube for later storage.
  4. Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.


  • Stock Solution Preparation for Adenosine
Total Mass [g] Stock Volume [mL] Concentration [M]
0.2006500.015


  • Serial Dilution of Adenosine Stock Solution
Dilution 'M1'[mol/L] 'V1' [mL] 'M2'[mol/L] 'V2' [mL]
10.0150.11.5E-410
20.0150.253.0E-510
31.5E-41.667 2.5E-510
41.5E-41.3332.0E-510
51.5E-41.01.5E-510
61.5E-40.6671.0E-510
71.5E-40.3330.5E-510
81.5E-40.50.75E-510

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