User:Moira M. Esson/Notebook/CHEM-571/2013/09/03: Difference between revisions
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# Prepare calibration curves for adenosine and inosine. | # Prepare calibration curves for adenosine and inosine. | ||
<br> | <br> | ||
==Preparation of Adenosine | ==Preparation of Adenosine Stock solution | ||
#In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H<sub>2</sub>O up until the white line. | |||
#Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved. | |||
#*'''Note''':The solution should be shaken vigorously for a minimum of 5 minutes. | |||
#Pour the solution into a 50mL falcon tube for later storage. | |||
#Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H<sub>2</sub>O. | |||
<br> | |||
* Stock Solution Preparation for Adenosine | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Total Mass [g]''' | |||
| align="center" style="background:#f0f0f0;"|'''Stock Volume [mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration ['''M''']''' | |||
|- | |||
| 0.2006||50||0.015||| | |||
|} | |||
<br> | |||
*Serial Dilution of Adenosine Stock Solution | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Dilution''' | |||
| align="center" style="background:#f0f0f0;"|''''''M<sub>1'''[mol/L]''' | |||
| align="center" style="background:#f0f0f0;"|''''''V<sub>1''' [mL]''' | |||
| align="center" style="background:#f0f0f0;"|''''''M<sub>2'''[mol/L]''' | |||
| align="center" style="background:#f0f0f0;"|''''''V<sub>2''' [mL]''' | |||
|- | |||
| 1||0.015||0.1||1.5E-4||10|| | |||
|- | |||
| 2||0.015||0.25||3.0E-5||10|| | |||
|- | |||
| 3||1.5E-4||1.667 ||2.5E-5||10|| | |||
|- | |||
| 4||1.5E-4||1.333||2.0E-5||10|| | |||
|- | |||
| 5||1.5E-4||1.0||1.5E-5||10|| | |||
|- | |||
| 6||1.5E-4||0.667||1.0E-5||10|| | |||
|- | |||
| 7||1.5E-4||0.333||0.5E-5||10|| | |||
|- | |||
| 8||1.5E-4||0.5||0.75E-5||10|| | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 11:16, 3 September 2013
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