User:Moira M. Esson/Notebook/CHEM-571/2013/09/03: Difference between revisions
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# Prepare inosine solutions of various concentrations to determine the molar absorptivity of [http://en.wikipedia.org/wiki/Inosine inosine]. | # Prepare inosine solutions of various concentrations to determine the molar absorptivity of [http://en.wikipedia.org/wiki/Inosine inosine]. | ||
# Prepare calibration curves for adenosine and inosine. | # Prepare calibration curves for adenosine and inosine. | ||
# Analyze the data of all of the combined CHEM-571 data. | |||
<br> | <br> | ||
==Preparation of Adenosine Stock solution== | ==Preparation of Adenosine Stock solution== | ||
General Protocol | General Protocol | ||
Line 76: | Line 78: | ||
==Preparation of Inosine Stock Solutions== | ==Preparation of Inosine Stock Solutions== | ||
General Protocol | |||
#In a clean 25mL volumetric flask, add 0.1347g inosine. Fill the volumetric flask with deionized H<sub>2</sub>O up until the white line. | |||
#*'''Note''': To ensure the full addition of all of the inosine, a small amount of deionized H<sub>2</sub>O was placed on the weigh boat and a pipette was used to remove all of the H<sub>2</sub>O on the weigh boat.s | |||
#Cap the volumetric flask and shake the volumetric flask vigorously until the inosine is completely dissolved. | |||
#*'''Note''':The solution should be shaken vigorously for a minimum of 3 minutes. | |||
#Pour the solution into a 50mL falcon tube for later storage. | |||
#Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H<sub>2</sub>O. | |||
<br> | |||
*Needed Concentrations for Inosine Solutions | |||
{|style="width:700px" | |||
|<u>Inosine solution concentrations (M)</u> | |||
|- | |||
|4.80x10<sup>-5</sup> | |||
|- | |||
|4.00x10<sup>-5</sup> | |||
|- | |||
|3.20x10<sup>-5</sup> | |||
|- | |||
|2.40x10<sup>-5</sup> | |||
|- | |||
|1.60x10<sup>-5</sup> | |||
|- | |||
|0.80x10<sup>-5</sup> | |||
|- | |||
|RANDOM | |||
|} | |||
<br> | |||
<br> | |||
* Stock Solution Preparation for Inosine | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Total Mass [g]''' | |||
| align="center" style="background:#f0f0f0;"|'''Stock Volume [mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration ['''M''']''' | |||
|- | |||
| 0.1347||25||0.02||| | |||
|} | |||
<br> | |||
*Serial Dilution of Inosine Stock Solution | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Dilution''' | |||
| align="center" style="background:#f0f0f0;"|''''''M<sub>1'''[mol/L]''' | |||
| align="center" style="background:#f0f0f0;"|''''''V<sub>1''' [mL]''' | |||
| align="center" style="background:#f0f0f0;"|''''''M<sub>2'''[mol/L]''' | |||
| align="center" style="background:#f0f0f0;"|''''''V<sub>2''' [mL]''' | |||
|- | |||
| 1||0.02||0.24||4.8E-4||10|| | |||
|- | |||
| 2||4.8E-4||1.0||4.8E-5||10|| | |||
|- | |||
| 3||4.8E-4||0.833||4.0E-5||10|| | |||
|- | |||
| 4||4.8E-4||0.667||3.2E-5||10|| | |||
|- | |||
| 5||4.8E-4||0.5||2.4E-5||10|| | |||
|- | |||
| 6||4.8E-4||0.333||1.6E-5||10|| | |||
|- | |||
| 7||4.8E-4||0.1667||0.8E-5||10|| | |||
|- | |||
| 8||1.5E-4||0.25||1.2E-5||10|| | |||
|} | |||
<br> | |||
==UV/vis== | |||
'''Figure 1. Absorbance of Standard Adenosine Solutions''' | |||
<br> | |||
[[Image:Correctedabsadenosine09042013rerunzem.png]] | |||
<br> | |||
*Maximum absorbance occurred at 260nm for each adenosine concentration | |||
'''Figure 2. Calibration Curve of Adenosine Solutions''' | |||
<br> | |||
[[Image:Calibrationcurveadenosinezem09082013.png]] | |||
<br> | |||
'''Figure 3. Absorbance of Standard Inosine Solutions''' | |||
<br> | |||
[[Image:Correctedabsinosinezem09042013.png]] | |||
<br> | |||
*The maximum absorbance of each solution occurred at 249nm. | |||
'''Figure 4. Calibration Curve of Inosine Solutions''' | |||
<br> | |||
[[Image:Calibrationcurveinosinestdszem09082013.png]] | |||
==Notes== | ==Notes== | ||
*Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed | *Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed thoroughly with deionized H<sub>2</sub>O. | ||
*Adenosine samples of 1.0E-5M and 3.0E-5M were re-prepared and re-run because these points were outliers on our original calibration curve. | |||
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|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 19:10, 8 September 2013
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Objectives
Preparation of Adenosine Stock solutionGeneral Protocol
Preparation of Inosine Stock SolutionsGeneral Protocol
UV/visFigure 1. Absorbance of Standard Adenosine Solutions
Figure 2. Calibration Curve of Adenosine Solutions
Figure 4. Calibration Curve of Inosine Solutions
Notes
|