User:Moira M. Esson/Notebook/CHEM-571/2013/08/28

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==Objectives==
==Objectives==
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#Prepare a bovine serum albumin (referred to as BSA) and gold nanoparticle (referred to as AuNP) solution for later testing.
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#Prepare a sodium citrate- AuNP solution.
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#Determine the concentration of the prepared sodium citrate-AuNP solution using UV/vis.
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<br>
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==BSA-AuNP Preparation==
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*The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/08/28|Dr.Harting's AU-Biomaterials Design Lab Notebook]] and [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference].
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<br>
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General Protocol:
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#Add 1mL 2.54mM HAuCl<sub>4</sub> solution prepared by Dr. Hartings to an 10mL volumetric flask.
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#Add 1.80mL 15.6μM BSA solution to the 10mL volumetric flask.(The concentration of gold in the solution is 90x that of the BSA)
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#Add 7.2mL deionized H<sub>2</sub>O to the 10mL volumetric flask.
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#Transfer the solution into a test tube. Cap the test tube with aluminum foil
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#Heat in oven at 80°C for 3hrs.
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#Store the solution in a blue falcon tube.
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'''Note''':Use caution when removing the test tube from oven. The test tube will be hot.
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<br>
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==Sodium citrate-AuNP solution Preparation==
 +
*The following protocol was taken from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/08/28|Dr.Harting's AU-Biomaterials Design Lab Notebook]] and [http://pubs.acs.org/doi/pdf/10.1021/ac0702084 this reference].
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<br>
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General Protocol:
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#Add 50mL of the prepared 0.295mM HAuCl<sub>4</sub> solution to a clean 150mL beaker.
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#Heat this solution to a boil while stirring the solution with a magnetic stir bar. Keep the beaker covered with a watch glass while the solution is heating.
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#After the solution comes to a boil, add 1.5mL of 1%(w/v) sodium citrate(prepared by Dr. Harting's).
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#Cover the beaker with a watch glass and allow to boil and stir for another 40 minutes.
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#Remove the beaker from the hot plate and allow to cool to room temperature. Keep the watch glass on the top of the beaker until solution is cooled.
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#Measure the volume of the solution.
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Prepared citrate-AuNP particle solution'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Final volume(mL)||||9.5
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|-
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| Final concentration citrate(M)||||0.0054
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|-
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| Final concentration Au(M)||||0.0013
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|}
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<br>
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==UV/vis analysis==
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Revision as of 22:26, 28 August 2013

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Objectives

  1. Prepare a bovine serum albumin (referred to as BSA) and gold nanoparticle (referred to as AuNP) solution for later testing.
  2. Prepare a sodium citrate- AuNP solution.
  3. Determine the concentration of the prepared sodium citrate-AuNP solution using UV/vis.


BSA-AuNP Preparation


General Protocol:

  1. Add 1mL 2.54mM HAuCl4 solution prepared by Dr. Hartings to an 10mL volumetric flask.
  2. Add 1.80mL 15.6μM BSA solution to the 10mL volumetric flask.(The concentration of gold in the solution is 90x that of the BSA)
  3. Add 7.2mL deionized H2O to the 10mL volumetric flask.
  4. Transfer the solution into a test tube. Cap the test tube with aluminum foil
  5. Heat in oven at 80°C for 3hrs.
  6. Store the solution in a blue falcon tube.

Note:Use caution when removing the test tube from oven. The test tube will be hot.

Sodium citrate-AuNP solution Preparation


General Protocol:

  1. Add 50mL of the prepared 0.295mM HAuCl4 solution to a clean 150mL beaker.
  2. Heat this solution to a boil while stirring the solution with a magnetic stir bar. Keep the beaker covered with a watch glass while the solution is heating.
  3. After the solution comes to a boil, add 1.5mL of 1%(w/v) sodium citrate(prepared by Dr. Harting's).
  4. Cover the beaker with a watch glass and allow to boil and stir for another 40 minutes.
  5. Remove the beaker from the hot plate and allow to cool to room temperature. Keep the watch glass on the top of the beaker until solution is cooled.
  6. Measure the volume of the solution.


Prepared citrate-AuNP particle solution ' '
Final volume(mL)9.5
Final concentration citrate(M)0.0054
Final concentration Au(M)0.0013


UV/vis analysis


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