User:Moira M. Esson/Notebook/CHEM-571/2013/08/28: Difference between revisions
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==UV/vis analysis== | ==UV/vis analysis== | ||
Figure 1. | *In order to determine the concentration of the solution, the solution was diluted using a 1/10 dilution. 1mL was used for the UV/vis sample. | ||
*To analyze the solution [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf this reference] was used. | |||
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'''Figure 1.''' | |||
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[[Image:Absorbance 10dilution CHEM571 08282013.png]] | [[Image:Absorbance 10dilution CHEM571 08282013.png]] | ||
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*The maximum absorbance of the citrate-AuNP was 0.405 at 518-521nm. | |||
*This wavelength range did not correspond to the supporting reference. This may indicate that too little gold or the incorrect amount of the citrate-AuNP solution was added to the 1/10 dilution. Another dilution will be run during the next meeting in order to determine the concentration of the prepared citrate-AuNP solution and the size of the diameter of the Au particles in the solution. | |||
==Notes== | |||
*After the addition of the citrate to the HAuCl<sub>4</sub> solution, the solution turned a light purple color. This color darkened during the 40min boiling process. Half way through the boiling process the solution turned a deep reddish purple color for approximately 5 min and then changed back to a dark purple color. | |||
*After the BSA-AuNP solution was heated for 3 hours, the solution turned into a dark purple color. | |||
Revision as of 19:42, 28 August 2013
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Objectives
BSA-AuNP Preparation
Note:Use caution when removing the test tube from oven. The test tube will be hot.
Sodium citrate-AuNP solution Preparation
UV/vis analysis
Notes
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