User:Moira M. Esson/Notebook/CHEM-571/2013/08/28: Difference between revisions
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| Final volume(mL)||9.5 | | Final volume(mL)||9.5 | ||
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*Added 0.01475mmol gold. With final volume of 9.5mL. concentration of gold is '''1.55mM'''. | |||
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==UV/vis analysis== | ==UV/vis analysis== | ||
*In order to determine the concentration of the solution, the solution was diluted using a 1/10 dilution. 1mL was used for the UV/vis sample. | |||
*To analyze the solution [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf this reference] was used. | |||
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'''Figure 1.''' | |||
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[[Image:Absorbance 10dilution CHEM571 08282013.png]] | |||
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*The maximum absorbance of the citrate-AuNP was 0.405 at 518-521nm. | |||
*The absorbance at 450nm was 0.259. | |||
*According to [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf this reference], the Au nano-particles have a diameter of 12nm. | |||
*The molar absorptivity coefficent for the Au-Citrate solution is 1.09E08 M<sup>-1</sup>cm<sup>-1</sup> | |||
*Using Beer's law, concentration of the 1/10 dilution was 2.38E-9M. | |||
[[User:Matt Hartings|Matt Hartings]] Moira, keep reading in this reference. There are notes on what to do if the size is less than 32nm. Please complete this analysis. You need to determine the size AND concentration of the nanoparticles along with the concentration of gold (based on your initial concentration and final volume) | |||
==Notes== | |||
*After the addition of the citrate to the HAuCl<sub>4</sub> solution, the solution turned a light purple color. This color darkened during the 40min boiling process. Half way through the boiling process the solution turned a deep reddish purple color for approximately 5 min and then changed back to a dark purple color. | |||
*After the BSA-AuNP solution was heated for 3 hours, the solution turned into a dark purple color. | |||
Revision as of 10:40, 17 September 2013
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Objectives
BSA-AuNP Preparation
Note:Use caution when removing the test tube from oven. The test tube will be hot.
Sodium citrate-AuNP solution Preparation
UV/vis analysis
Notes
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