Objective 1) Swelling Studies of PVOH film with 1ml glutaraldehyde with a molar mass of MW=22,000 g/mol.
Objective 2) 5 - (4,6-dichlorotriazinyl) aminofluoresceine (5-DTFA) Dye Attachment studies.
- Swelling Studies:
- mass of PVOH film with 1 ml glutaraldehyde = 0.6707g. Note: The film was dried effectively before it was weigh.
- Dye Attachment
- mass of PVOH = 0.500 g was placed in a small, 10 ml beaker
- MW of PVOH = 22,000 g/mol
- PVOH was then dissolved in 8 mL of 0.1M NaHCO3 (sodium bicarbonate) buffered solution (at a pH of around 10) for successful deprotination of the dye.
- The entire solution was then heated and stirred at until all of the dye was dissolved
- After that about 1 mg of the dye was added to the resultant solution
- The entire solution was heated up to 1 hour
- Finally, the solution was poured into the big teflon plate and was stored for about 1 week.
- Add data and results here...
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