User:Mobeen Ashraf/Notebook/Chem-581/2012/11/02

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Current revision (18:18, 30 November 2012) (view source)
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==Description==
==Description==
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The following sulfate/porphyrin solutions were prepared and the absorbance was measured for each one between 200 and 800 nm by using UV/VIS instrument:
+
Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:
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1) 1mL 50μM Na2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
+
* 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used
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2) 1mL 50μM Fe2(SO4)3, 1mL 5μM porphyrin, 2 mL HPLC H2O
+
* 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
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3) 1mL 50μM FeSO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
+
* 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
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4) 1mL 50μM H2SO4, 1mL 5μM porphyrin, 2 mL HPLC H2O
+
* 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
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5) 1mL 50μM Al2(SO4)3
+
* 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.
==Data==
==Data==

Current revision

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Objective

  • Make 1:1 ratio of sulfate ions solution ( H2SO4, Fe2(SO4)3, FeSO4, Na2SO4, Al2(SO4)3).
  • Adsorption through films similar to clay films instead of clay :
    • 1.04 g Fe(II) Cl, 0.5 g Iron Sulfate, 1 film, and 40 ml H2O.
    • To precipitate iron oxide out of the solution NaOH ( 2.67 g in 10 ml H2O) was added drop wise with stirring.
    • The solution was then filtered using vacuum filtration and left to dry.
  • Add film with Porphyrin and glutaraldehyde to 1% solution of HCl and leave out until the next working day.

Description

Sulfate (SO4 2-) as generated by the following solutions along with the porphyrin solution were prepared. The absorbance was measured for each one between by using the UV-Vis instrument by Shimadzu and the selected wavelength range in nm was [200 nm- 800 nm]:

  • 1mL of 50μM Na2SO4 was dissolved in a 1mL of 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM Fe2(SO4)3 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL of 50μM FeSO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM H2SO4 was dissolved in a 1mL, 5μM porphyrin solution and 2 mL HPLC graded water was used
  • 1mL 50μM Al2(SO4)3 was also dissolved in a 1mL of a porphyrin solution and 2 mL HPLC graded water was used.

Data

Image:UV-VIS_Curve_for_Porphyrin_sulfate_solutions..png

Notes

Note: The graph in the data section is a courtesy of Allison Alix.



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