User:Mobeen Ashraf/Notebook/Chem-581/2012/10/24: Difference between revisions
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==Description== | ==Description== | ||
* | *Porphyrin cross-linking was done again by placing 2 drops of H2SO4 and it was cross-linked with out Na2SO4. To do that, 2 drops of HCl, 1 ml of glutaraldeyde, ml of porphyrin was used. | ||
* | |||
* UV-VIS of Porphyrin Solutions: | |||
100 micro molar, 50 micro molar, 25 micro molar, 12.5 micro molar, and 6.75 micro molar porphyrin dilutions were made for UV-VIS analysis. | |||
3.375 micro molar and 1.6875 micro molar solution of porphyrin solutions were made. | |||
* 3 stock solutions of 50 micro molar sodium sulfate (Na2SO4), Iron (II) sulfate (FeSO4) and aluminium sulfate (Al2(SO4)3) were prepared. | |||
50 micro molar solution for Na2SO4 was prepared in a following stoichiometric way: | |||
Molecular Weight= 142.07g/mol | |||
(142.07g/mol)* ( 50 μ mol/1L)* (0.000001mol/1μ mol)* (1L/1,000 ml) * 10 ml = 0.071035 g in 10 ml of HPLC graded water. | |||
We lowered the concentration down to 50 m M (milli molar) in a following way: | |||
(50 mM) V1= (50 μΜ) (10 mL) | |||
V1=0.01 mL. Therefore, 10 μL in 9.99 ml (999 μL) was added to make 50 mM Na2SO4 solution. | |||
Note: The same procedure was employed when making 50μM and 50 mM solutions of FeSO4 and Al2(SO4)3. 0.144 g of Fe2(SO4)3 was added in 10 ml of HPLC water to make 50 mM solution and 0.328 g of Al2(SO4)3 was added in 10 ml of HPLC graded water to make 50 mM solution. | |||
==Data== | ==Data== |
Revision as of 16:37, 8 November 2012
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ObjectiveObjective 1) Porphyrin Cross-Linking 9again) with 2 drops of H2SO4 and cross link with out Na2SO4. Objective 2) Perform Porphyrin dilutions Objective 3) UV-Vis analysis of porphyrin dilutions. Description
100 micro molar, 50 micro molar, 25 micro molar, 12.5 micro molar, and 6.75 micro molar porphyrin dilutions were made for UV-VIS analysis. 3.375 micro molar and 1.6875 micro molar solution of porphyrin solutions were made.
50 micro molar solution for Na2SO4 was prepared in a following stoichiometric way: Molecular Weight= 142.07g/mol (142.07g/mol)* ( 50 μ mol/1L)* (0.000001mol/1μ mol)* (1L/1,000 ml) * 10 ml = 0.071035 g in 10 ml of HPLC graded water. We lowered the concentration down to 50 m M (milli molar) in a following way: (50 mM) V1= (50 μΜ) (10 mL) V1=0.01 mL. Therefore, 10 μL in 9.99 ml (999 μL) was added to make 50 mM Na2SO4 solution. Note: The same procedure was employed when making 50μM and 50 mM solutions of FeSO4 and Al2(SO4)3. 0.144 g of Fe2(SO4)3 was added in 10 ml of HPLC water to make 50 mM solution and 0.328 g of Al2(SO4)3 was added in 10 ml of HPLC graded water to make 50 mM solution. Data
NotesThis area is for any observations or conclusions that you would like to note.
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