User:Mischa L Spiegel/Notebook/Does DMSP have any effect on angiogenesis/: Difference between revisions

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==Introduction==
==Introduction==
* Observed and attempted removing tomato mouse endothelial cells (transformed with RFP)from cell culture
* Observed and attempted removing tomato mouse endothelial cells (transformed with RFP)from cell culture
* 30µl trypan blue added to 30µl cell culture and counted in haemocytometer  
* 30µl trypan blue added to 30µl cell culture and counted in haemocytometer = 2.3 x 10^5/ml cells
* 2.3 x 10^5/ml cells
* Portion of cells put back into cell culture  
* Portion of cells put back into cell culture  
* BCA assay conducted
* Western gel made




Latest revision as of 01:02, 27 September 2017

Project name Main project page

Introduction

  • Observed and attempted removing tomato mouse endothelial cells (transformed with RFP)from cell culture
  • 30µl trypan blue added to 30µl cell culture and counted in haemocytometer = 2.3 x 10^5/ml cells
  • Portion of cells put back into cell culture
  • BCA assay conducted
  • Western gel made


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