User:Mike Barnkob

Information

• Mike Barnkob
• Weatherall Institute for Molecular Medicine, University of Oxford
• Website: www.mikebarnkob.dk.

Protocols

Coloning

• CRISPR / Cas9
  • Designing sgRNA oligos
  • Cloning oligos into backbone
  • Transforming bacteria with construct
  • Screening for inserts
• Lentiviral work
  • Lentiviral production
  • Infecting target cells

Bacterial work

• Making LB agar plates

Immunology

• Flow cytometry
• CFSE staining

Tissue culture work

• Medium
  • R10
  • D10
  • DMEM for 293TLA cells
• Thawing cells
• Freezing down cells
• Split cells
• Count cells
• Harvest splenocytes
• Harvest BMDCs
• In vitro expansion of T cells
• Soft agar assay

Mouse work

• Bleeding
• Harvesting bone-marrow DC's

Visualization

• Long-time co-culture and visualization with live-dead marker

Projects

I work on:

• T cell and cancer cell interactions
• Genetic manipulation of T cells

Past:

[[Mike_Barnkob:Projects/