User:Mike Barnkob: Difference between revisions
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** [[Mike_Barnkob:Protocols/Cloning/Isolating_mRNA_from_spleen|Isolating mRNA from spleen]] | ** [[Mike_Barnkob:Protocols/Cloning/Isolating_mRNA_from_spleen|Isolating mRNA from spleen]] | ||
** [[Mike_Barnkob_Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]] | ** [[Mike_Barnkob_Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]] | ||
** [[Mike_Barnkob:Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]] | |||
** [[Mike_Barnkob:Protocols/Cloning/PCR|PCR]] | |||
* CRISPR / Cas9 | * CRISPR / Cas9 | ||
** Designing sgRNA oligos | ** Designing sgRNA oligos | ||
| Line 45: | Line 47: | ||
* ''In vitro'' expansion of T cells | * ''In vitro'' expansion of T cells | ||
* Soft agar assay | * Soft agar assay | ||
Visualization | Visualization | ||
Revision as of 03:17, 28 January 2015
Information
- Mike Barnkob
- Website: www.mikebarnkob.dk.
Protocols
Cloning
- Basics
- CRISPR / Cas9
- Designing sgRNA oligos
- Cloning oligos into backbone
- Transforming bacteria with construct
- Screening for inserts
- Lentiviral work
- Lentiviral production
- Infecting target cells
Bacterial work
- Prepare antibiotics
- LB agar plates
- Streaking plates
- Inoculate overnight liquid culture
- Glycerol stock
- Isolate plasmid DNA
- Diagnostic Restriction Digest
Immunology
- Flow cytometry
- CFSE staining
Tissue culture work
- Media
- Thawing cells
- Freezing down cells
- Split cells
- Count cells
- Harvest splenocytes
- Harvest BMDCs
- In vitro expansion of T cells
- Soft agar assay
Visualization
- Long-time co-culture and visualization with live-dead marker
Projects
I work on:
- T cell and cancer cell interactions
- Genetic manipulation of T cells
Past:
Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.
Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).
