User:Mike Barnkob: Difference between revisions

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==Contact Info==
==Information==
[[Image:OWWEmblem.png|thumb|right|Mike Barnkob (an artistic interpretation)]]


*Mike Barnkob
*Mike Barnkob
*Faculty of Health
*Website: [http://www.mikebarnkob.dk www.mikebarnkob.dk].
*University of Southern Denmark
*[[Special:Emailuser/Mike Barnkob|Email me through OpenWetWare]]


==Education==
==Protocols==


* 2012, MD, Faculty of Health, University of Southern Denmark
Cloning
* 2009, B.Sc.med, Faculty of Health, University of Southern Denmark
* Basics
** [[Mike_Barnkob:Protocols/Cloning/Solutions|Solutions]]
** [[Mike_Barnkob:Protocols/Cloning/Primer_Design|Primer design]]
** [[Mike_Barnkob:Protocols/Cloning/Gels|Gels]]
** [[Mike_Barnkob:Protocols/Cloning/Isolating_mRNA_from_spleen|Isolating mRNA from spleen]]
** [[Mike_Barnkob_Protocols/Cloning/cDNA_library_from_mRNA|Creating cDNA from mRNA]]
* CRISPR / Cas9
** Designing sgRNA oligos
** Cloning oligos into backbone
** Transforming bacteria with construct
** Screening for inserts
* Lentiviral work
** Lentiviral production
** Infecting target cells


==Research interests==
Bacterial work
* [[Mike_Barnkob:Protocols/Bacterial/Antibiotics|Prepare antibiotics]]
* [[Mike_Barnkob:Protocols/Bacterial/LB_agar_plates|LB agar plates]]
* [[Mike_Barnkob:Protocols/Bacterial/Streaking|Streaking plates]]
* [[Mike_Barnkob:Protocols/Bacterial/ON_liquid_culture|Inoculate overnight liquid culture]]
* [[Mike_Barnkob:Protocols/Bacterial/Glycerol_stock|Glycerol stock]]
* [[Mike_Barnkob:Protocols/Bacterial/Isolate DNA|Isolate plasmid DNA]]
* [[Mike_Barnkob:Protocols/Bacterial/Diagnostic_Restriction_Digest|Diagnostic Restriction Digest]]


# Synthetic biology
Immunology
# Cancer research
* Flow cytometry
# Lab automatation
* CFSE staining


==Publications==
Tissue culture work
* [[Mike_Barnkob:Protocols/Mediums|Media]]
* [[Mike_Barnkob:Protocols/Tissue_Culture/Thawing cells|Thawing cells]]
* Freezing down cells
* Split cells
* Count cells
* Harvest splenocytes
* Harvest BMDCs
* ''In vitro'' expansion of T cells
* Soft agar assay


Mouse work
* Bleeding
* Harvesting bone-marrow DC's


==Useful links==
Visualization
*[[OpenWetWare:Welcome|Introductory tutorial]]
* Long-time co-culture and visualization with live-dead marker
*[[Help|OpenWetWare help pages]]
 
==Projects==
 
I work on:
* T cell and cancer cell interactions
* Genetic manipulation of T cells
 
'''Past:'''
 
[[Mike_Barnkob:Projects/Liquid handling robot|Liquid handling robot]]. An attempt to create a small liquid handling robot to do simple experiments with.
 
[http://2009.igem.org/Team:SDU-Denmark Bacto Bandage]. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).

Revision as of 04:38, 22 January 2015

Information

Protocols

Cloning

Bacterial work

Immunology

  • Flow cytometry
  • CFSE staining

Tissue culture work

  • Media
  • Thawing cells
  • Freezing down cells
  • Split cells
  • Count cells
  • Harvest splenocytes
  • Harvest BMDCs
  • In vitro expansion of T cells
  • Soft agar assay

Mouse work

  • Bleeding
  • Harvesting bone-marrow DC's

Visualization

  • Long-time co-culture and visualization with live-dead marker

Projects

I work on:

  • T cell and cancer cell interactions
  • Genetic manipulation of T cells

Past:

Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.

Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).

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