User:Mike Barnkob: Difference between revisions
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Tissue culture work | Tissue culture work | ||
* Medium | |||
** R10 | |||
** D10 | |||
** DMEM for 293TLA cells | |||
* Thawing cells | |||
* Freezing down cells | |||
* Split cells | * Split cells | ||
* Count cells | * Count cells | ||
| Line 31: | Line 37: | ||
* Harvest BMDCs | * Harvest BMDCs | ||
* ''In vitro'' expansion of T cells | * ''In vitro'' expansion of T cells | ||
* Soft agar assay | |||
Mouse work | Mouse work | ||
* Bleeding | * Bleeding | ||
* Harvesting bone-marrow DC's | |||
Visualization | |||
* Long-time co-culture and visualization with live-dead marker | |||
==Projects== | ==Projects== | ||
Revision as of 03:52, 18 December 2014
Information
- Mike Barnkob
- Weatherall Institute for Molecular Medicine, University of Oxford
- Website: www.mikebarnkob.dk.
Protocols
Coloning
- CRISPR / Cas9
- Designing sgRNA oligos
- Cloning oligos into backbone
- Transforming bacteria with construct
- Screening for inserts
- Lentiviral work
- Lentiviral production
- Infecting target cells
Bacterial work
- Making LB agar plates
Immunology
- Flow cytometry
- CFSE staining
Tissue culture work
- Medium
- R10
- D10
- DMEM for 293TLA cells
- Thawing cells
- Freezing down cells
- Split cells
- Count cells
- Harvest splenocytes
- Harvest BMDCs
- In vitro expansion of T cells
- Soft agar assay
Mouse work
- Bleeding
- Harvesting bone-marrow DC's
Visualization
- Long-time co-culture and visualization with live-dead marker
Projects
I work on:
- T cell and cancer cell interactions
- Genetic manipulation of T cells
Past:
[[Mike_Barnkob:Projects/
