User:Mike Barnkob: Difference between revisions

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I'm medical student from Denmark, with interests in microbiology, genetics and ways to engineer human cells.
==Information==


==Contact Info==
*Mike Barnkob
[[Image:OWWEmblem.png|thumb|right|Mike Barnkob (an artistic interpretation)]]
*Weatherall Institute for Molecular Medicine, University of Oxford
*Website: [http://www.mikebarnkob.dk www.mikebarnkob.dk].
 
==Protocols==
 
Coloning
* CRISPR / Cas9
** Designing sgRNA oligos
** Cloning oligos into backbone
** Transforming bacteria with construct
** Screening for inserts
* Lentiviral work
** Lentiviral production
** Infecting target cells


*Mike Barnkob
Bacterial work
*Faculty of Health, University of Southern Denmark
* Making LB agar plates
*Email: [[Special:Emailuser/Mike Barnkob|Say hallo]].
*  
*Blog: [http://www.mikebarnkob.dk www.mikebarnkob.dk].


==Education==
Immunology
* Flow cytometry
* CFSE staining


* 2012, MD, Faculty of Health, University of Southern Denmark
Tissue culture work
* 2009, B.Sc.med, Faculty of Health, University of Southern Denmark
* Medium
** R10
** D10
** DMEM for 293TLA cells
* Thawing cells
* Freezing down cells
* Split cells
* Count cells
* Harvest splenocytes
* Harvest BMDCs
* ''In vitro'' expansion of T cells
* Soft agar assay


==Research interests==
Mouse work
* Bleeding
* Harvesting bone-marrow DC's


# Synthetic biology
Visualization
# Cancer research
* Long-time co-culture and visualization with live-dead marker
# Lab automation


==Projects==
==Projects==


'''Current:'''
I work on:
 
* T cell and cancer cell interactions
Open source liquid handling robot.
* Genetic manipulation of T cells


'''Past:'''
'''Past:'''


[http://2009.igem.org/Team:SDU-Denmark Bacto Bandage]. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).
[[Mike_Barnkob:Projects/
 
==Publications==

Revision as of 03:52, 18 December 2014

Information

  • Mike Barnkob
  • Weatherall Institute for Molecular Medicine, University of Oxford
  • Website: www.mikebarnkob.dk.

Protocols

Coloning

  • CRISPR / Cas9
    • Designing sgRNA oligos
    • Cloning oligos into backbone
    • Transforming bacteria with construct
    • Screening for inserts
  • Lentiviral work
    • Lentiviral production
    • Infecting target cells

Bacterial work

  • Making LB agar plates

Immunology

  • Flow cytometry
  • CFSE staining

Tissue culture work

  • Medium
    • R10
    • D10
    • DMEM for 293TLA cells
  • Thawing cells
  • Freezing down cells
  • Split cells
  • Count cells
  • Harvest splenocytes
  • Harvest BMDCs
  • In vitro expansion of T cells
  • Soft agar assay

Mouse work

  • Bleeding
  • Harvesting bone-marrow DC's

Visualization

  • Long-time co-culture and visualization with live-dead marker

Projects

I work on:

  • T cell and cancer cell interactions
  • Genetic manipulation of T cells

Past:

[[Mike_Barnkob:Projects/

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