User:Mike Barnkob: Difference between revisions
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== | ==Information== | ||
*Mike Barnkob | *Mike Barnkob | ||
* | *Weatherall Institute for Molecular Medicine, University of Oxford | ||
*Website: [http://www.mikebarnkob.dk www.mikebarnkob.dk]. | |||
*[ | |||
== | ==Protocols== | ||
* | Coloning | ||
* | * CRISPR / Cas9 | ||
** Designing sgRNA oligos | |||
** Cloning oligos into backbone | |||
** Transforming bacteria with construct | |||
** Screening for inserts | |||
* Lentiviral work | |||
** Lentiviral production | |||
** Infecting target cells | |||
Bacterial work | |||
* Making LB agar plates | |||
* | |||
Immunology | |||
* Flow cytometry | |||
* CFSE staining | |||
Tissue culture work | |||
* Medium | |||
** R10 | |||
** D10 | |||
** DMEM for 293TLA cells | |||
* Thawing cells | |||
* Freezing down cells | |||
* Split cells | |||
* Count cells | |||
* Harvest splenocytes | |||
* Harvest BMDCs | |||
* ''In vitro'' expansion of T cells | |||
* Soft agar assay | |||
Mouse work | |||
* Bleeding | |||
* Harvesting bone-marrow DC's | |||
== | Visualization | ||
* | * Long-time co-culture and visualization with live-dead marker | ||
==Projects== | |||
I work on: | |||
* T cell and cancer cell interactions | |||
* Genetic manipulation of T cells | |||
'''Past:''' | |||
[[Mike_Barnkob:Projects/ | |||
Revision as of 03:52, 18 December 2014
Information
- Mike Barnkob
- Weatherall Institute for Molecular Medicine, University of Oxford
- Website: www.mikebarnkob.dk.
Protocols
Coloning
- CRISPR / Cas9
- Designing sgRNA oligos
- Cloning oligos into backbone
- Transforming bacteria with construct
- Screening for inserts
- Lentiviral work
- Lentiviral production
- Infecting target cells
Bacterial work
- Making LB agar plates
Immunology
- Flow cytometry
- CFSE staining
Tissue culture work
- Medium
- R10
- D10
- DMEM for 293TLA cells
- Thawing cells
- Freezing down cells
- Split cells
- Count cells
- Harvest splenocytes
- Harvest BMDCs
- In vitro expansion of T cells
- Soft agar assay
Mouse work
- Bleeding
- Harvesting bone-marrow DC's
Visualization
- Long-time co-culture and visualization with live-dead marker
Projects
I work on:
- T cell and cancer cell interactions
- Genetic manipulation of T cells
Past:
[[Mike_Barnkob:Projects/
