User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2016/04/08: Difference between revisions

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==Entry title==
==Run Temperature Trials==
* Insert content here...
* I came in today and added Rhodamine B to 18 samples such that the 10 mL fiber samples all had Rhodamine B concentrations of 10 μM. See Nicole's notebook from Tuesday for volumes and concentrations used. 9 of these samples were then placed in an ice water bath (0 °C) for 2 hours. The remaining 9 samples were placed in the oven at 60 degrees Celsius for 2 hours.
* Matt joined me two hours later and we spun down each sample, and pipetted off a 3 mL sample from each to fill cuvettes.  UV-Vis spectra were then taken as on March 30th. 
 
==Results==
 
*Data below are both raw and worked up UV-Vis data for 0°C and 60 °C samples:
 
[[Image:Zero_Degree_Data.png|500px]]
 
[[Image:60_Degree_Data_Table.png|500px]]
 
[[Image:0_Degree_Bar_Graph_avg_fixed.png|500px]]
 
The image above shows the percent change in Rhodamine B concentration after 2 hours incubating with fibers at 0 degrees Celsius. 
 
[[Image:60_Degree_Bar_Graph_black_avg.png|500px]]
 
The image above shows the percent change in Rhodamine B concentration after 2 hours incubating with fibers at 60 degrees Celsius. 





Latest revision as of 01:43, 27 September 2017

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Run Temperature Trials

  • I came in today and added Rhodamine B to 18 samples such that the 10 mL fiber samples all had Rhodamine B concentrations of 10 μM. See Nicole's notebook from Tuesday for volumes and concentrations used. 9 of these samples were then placed in an ice water bath (0 °C) for 2 hours. The remaining 9 samples were placed in the oven at 60 degrees Celsius for 2 hours.
  • Matt joined me two hours later and we spun down each sample, and pipetted off a 3 mL sample from each to fill cuvettes. UV-Vis spectra were then taken as on March 30th.

Results

  • Data below are both raw and worked up UV-Vis data for 0°C and 60 °C samples:

The image above shows the percent change in Rhodamine B concentration after 2 hours incubating with fibers at 0 degrees Celsius.

The image above shows the percent change in Rhodamine B concentration after 2 hours incubating with fibers at 60 degrees Celsius.