Objective
- Prepare Samples for ICP, UV-Vis, and Conductivity Analysis Tomorrow
Preparation of Gold Standard Solutions for ICP
- I created standard solutions of Gold for ICP analysis
- Standards were prepared using a serial dilution. Stock Au standard solution had a concentration of 1 mg/ml. From this, I created 6 additional standard solutions to make a calibration curve in ICP.
- In a 50 mL volumetric flask, I combined 5 mL of stock Au solution with DI water. This was Standard A. Concentration of Gold: 0.1 mg/mL
- I transferred 5 mL of Standard A to another 50 mL volumetric flask and filled it with DI water. This was Standard B. Concentration of Gold: 0.01 mg/mL
- I transferred 5 mL of Standard B to another 50 mL volumetric flask and filled it with DI water. This was Standard C. Concentration of Gold: 0.001 mg/mL
- I transferred 5 mL of Standard C to another 50 mL volumetric flask and filled it with DI water. This was Standard D. Concentration of Gold: 0.0001 mg/mL
- I transferred 5 mL of Standard D to another 50 mL volumetric flask and filled it with DI water. This was Standard E. Concentration of Gold: 0.00001 mg/mL
- I transferred 5 mL of Standard E to another 50 mL volumetric flask and filled it with DI water. This was Standard F. Concentration of Gold: 0.000001 mg/mL
Preparation of Samples for Next Day Analysis
- Each group prepared 3 replicate samples for their particular protease at four different concentrations.
- Samples were prepared as follows:
- The supernatant from 20 mL AuNP Fiber solutions were pipetted off. Three of these supernatant samples were retained for analysis the next day.
- To the AuNP fiber samples, an appropriate volume of Tris buffer was added along with each protease to make protease concentrations of 1 μM, 100 nM, 10 nM, and 1 nM with a total volume of 20 mL.
- In addition, blank AuNP fiber samples were created such that no protease was added to the solution. Three of these blanks were created.
- The sample vials were incubated at 37°C overnight.
Notes
- Becky and I did not create our own protease samples as Matt's group was already running samples with Proteinase K. Instead, Becky and I focused our attention on helping the other groups prepare their samples, and on Wednesday we will be running the blanks, standards, blanks of lysozyme, chloroauric acid, and unreacted lysozyme and chloroauric acid.
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