User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/09/02: Difference between revisions
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== | ==Objective== | ||
* | |||
Prepare solutions of lysozyme/AuNP fibers. | |||
==Procedure== | |||
Stock solutions of HAuCl<sub>4</sub>·H<sub>2</sub>O and Lysozyme were prepared by Dr. Hartings. The procedure for their preparation can be found [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/02| here]]. | |||
The stock solution of HAuCl<sub>4</sub>·H<sub>2</sub>O has a concentration of 2.96 mM. The stock solution of Lysozyme has a concentration of 21.4 μM. | |||
Sixteen 1 mL samples and ten 5 mL samples of a 45:1 Au:Lysozyme solution were prepared using the following volumes of stock solutions: | |||
# 1 mL samples | |||
## Volume of gold stock: 84.4 μL | |||
## Volume of protein stock: 259 μL | |||
## Volume of water: 656 μL | |||
# 5 mL samples | |||
## Volume of gold stock: 422 μL | |||
## Volume of protein stock: 1,297 μL | |||
## Volume of water: 3,281 μL | |||
These solutions were then heated in the oven at 80°C for four hours. | |||
==Results== | |||
* All ten 5 mL solutions failed to produce nanofibers. | |||
* All sixteen 1 mL solutions failed to produce nanofibers. | |||
* All solutions that failed to produce nanofibers yielded solutions of nanoparticles. | |||
* Solutions of nanoparticles created by accident were stored in a 50 mL falcon tube should a need for them arise in the future of our study. | |||
Latest revision as of 01:07, 27 September 2017
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ObjectivePrepare solutions of lysozyme/AuNP fibers. ProcedureStock solutions of HAuCl4·H2O and Lysozyme were prepared by Dr. Hartings. The procedure for their preparation can be found here. The stock solution of HAuCl4·H2O has a concentration of 2.96 mM. The stock solution of Lysozyme has a concentration of 21.4 μM. Sixteen 1 mL samples and ten 5 mL samples of a 45:1 Au:Lysozyme solution were prepared using the following volumes of stock solutions:
These solutions were then heated in the oven at 80°C for four hours. Results
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