Objective
Today we conducted a diffusion test using a PVA film.
Description
Diffusion
- Our group used a PVA film
- Jake, Mary, and James used a PVA/clay film
- We prepared the diffusion chambers in the following manner
- Set the film between the two chambers
- Added liquid to each chamber
- One chamber was filled with 10 mL water
- The other chamber was filled with 10 mL 20ppm Malachite green
- Withdraw 200uL from each chamber at the 5 minute mark and place in their own eppy tube
- NOTE: I want the groups to coordinate so that their 5 minutes don't overlap with one another.
- NOTE: We used a small volume cuvette
- NOTE: We took a 0 minute measurement for each.
- NOTE: We had to dilute the sample from our chamber with the 20ppm. We used a 1:2 dilution each time. So, when we returned the sample, we only returned the non-diluted sample.
- We measured the UV-Vis spectrum for both samples and returned each to the chamber it originally came from
- We took measurements at the following times
- 6 minutes
- 17 minutes
- 30 minutes
- 1 hour
- 2 hours
- right before we left lab
General Protocol taken from Dr. Hartings.
Data
- Add data and results here...
Notes
Add notes here.
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