User:Michael S. Bible/Notebook/581/2014/09/19

Biomaterials Design Lab

Main project page Previous entry      Next entry

Film Absorbance of Arsenic and Mercury Today we set up an experiment similar to the malachite green experiment conducted earlier. Pieces of polymer and polymer+clay will be placed in vials containing solutions of As and Hg with concentrations of 50 ppm, 100 ppm, and 150 ppm. In total, there will be 12 vials of solution+films created Initial Film Masses Below is a chart containing the initial masses of each of the polymer and polymer+clay films used in the experiment. Creating Arsenic and Mercury Solutions To make 50, 100, and 150 ppm solutions of Mercury and Arsenic, the following calculations were done. V1*Conc.1=V2*Conc.2 Making 50 mL Hg solutions from Dr. Hartings's 268 ppm Hg stock solution: (50 mL)*(50ppm) = (x)*(268ppm) x = [(50 mL)*(50 ppm)]/(268ppm) =9.32 mL of Hg Stock Solution (50 mL)*(100ppm) = (x)*(268ppm) x = [(50 mL)*(100 ppm)]/(268ppm) =18.66 mL of Hg Stock Solution (50 mL)*(150ppm) = (x)*(268ppm) x = [(50 mL)*(150 ppm)]/(268ppm) =27.98 mL of Hg Stock Solution Making 50 mL As solutions from 0.0499 M Arsenite solution: Converting from M to ppm: (0.0499 mol Arsenite/L Arsenite)*(74.922 g Arsenic/mol of Arsenite)*(1000 mg Arsenic/1 g Arsenic) = 3738.61 mg/L = 3738.61 ppm (100 mL)*(50ppm) = (x)*(3738.61ppm) x = [(50 mL)*(50 ppm)]/(3738.61ppm) =4.00 mL of Arsenite Stock Solution (100 mL)*(100ppm) = (x)*(3738.61ppm) x = [(50 mL)*(100 ppm)]/(3738.61ppm) =2.76 mL of Arsenite Stock Solution (100 mL)*(150ppm) = (x)*(3738.61ppm) x = [(50 mL)*(150 ppm)]/(3738.61ppm) =1.32 mL of Arsenite Stock Solution Thanks to Eleni for creating the Arsenic solutions. Notes Add notes here.