User:Michael S. Bible/Notebook/581/2014/09/17: Difference between revisions

From OpenWetWare
< User:Michael S. Bible‎ | Notebook‎ | 581‎ | 2014‎ | 09
Jump to navigationJump to search
Line 34: Line 34:
**80ppm*0.5mL=8ppm*5mL(vial with no film)
**80ppm*0.5mL=8ppm*5mL(vial with no film)
**80ppm*0.2mL=8ppm*2mL(vial with PVA film)
**80ppm*0.2mL=8ppm*2mL(vial with PVA film)
==Figures==
Figure 1. Shows the separation of the clay after the first washing of water.
[[Image:washing1.png]]
Figure 2. Shows the dried clay being collected for future testing.
[[Image:dry.png]]
==Results==
==Results==


Revision as of 20:32, 23 September 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Description

  • Measure MG new data through UV-Vis
  • Film Processing
  • Filter stirring clay in centrifuge
    • 4000rpm for 30 minutes at 4°C for original separation
    • rinse with water then ethanol for 10 minutes
    • repeat for another cycle and drying done by Dr. Hartings

PXRD

  • Set up PXRD of PVA+Clay films at different concentrations.
    • Tray 1 -Clay powder-control
    • Tray 2 -8ppm by Madeleine
    • Tray 3 -2ppm by
    • Tray 4 -PVA film-control
    • Tray 5 -80ppm by Eleni
    • Tray 6 -200ppm by my group
      • Instrument Settings
        • 2o start angle
        • 40o stop angle
        • 1o/second scan speed (this seems too fast, will check on this)
        • 0.5o sampling width

Calculations

  • Dilutions of MG solutions
    • 200ppm*0.2mL=8ppm*5mL(vial with PVA film)
    • 80ppm*0.5mL=8ppm*5mL(vial with no film)
    • 80ppm*0.2mL=8ppm*2mL(vial with PVA film)

Figures

Figure 1. Shows the separation of the clay after the first washing of water.

Figure 2. Shows the dried clay being collected for future testing.

Results

UV-Vis was performed on the solutions of MG started on September 12 and the results can be found in the graph and chart below.

The concentration of MG in the film was calculated in the following way:


(Initial ppm)(.010L) = (Initial mass of MG in 10 mL of solution, mg)


[(Solution Absorbance post-experiment)/(molar abosorptivity of Malachite Green according to calibration curve from September 3rd = 0.1193/ppm)]x(Volume = 0.01L) = (Final mass of MG in solution, mg)


[(Initial mass of MG in Solution)-(Final mass of MG in solution, mg)]/(initial mass of film) = (Concentration of MG in film, mgMG/gFilm)

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_S._Bible/Notebook/581/2014/09/17&oldid=822703"