User:Michael S. Bible/Notebook/581/2014/09/17: Difference between revisions

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*Measure MG new data through UV-Vis
*Measure MG new data through UV-Vis
*Film Processing
*Film Processing
*Filter stirring clay in centriuge
*Filter stirring clay in centrifuge
**4000rpm for 30 minutes at 4C for original separation
**4000rpm for 30 minutes at 4°C for original separation
**with water then ethanol for 10 minutes  
**rinse with water then ethanol for 10 minutes  
**repeat for another cycle and dried done by Dr. Hartings
**repeat for another cycle and drying done by Dr. Hartings


==PXRD==
==PXRD==
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[[Image:ICE table PVA+control.png]]
[[Image:ICE table PVA+control.png]]
The concentration of MG in the film was calculated in the following way:
(Initial ppm)(.010L) = (Initial mass of MG in 10 mL of solution, mg)
[(Solution Absorbance post-experiment)/(molar abosorptivity of Malachite Green according to calibration curve from [[User:Michael_S._Bible/Notebook/581/2014/09/03| September 3rd]] = 0.1193/ppm)]x(Volume = 0.01L) = (Final mass of MG in solution, mg)
[(Initial mass of MG in Solution)-(Final mass of MG in solution, mg)]/(initial mass of film) = (Concentration of MG in film, mgMG/gFilm)

Revision as of 12:21, 19 September 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Description

  • Measure MG new data through UV-Vis
  • Film Processing
  • Filter stirring clay in centrifuge
    • 4000rpm for 30 minutes at 4°C for original separation
    • rinse with water then ethanol for 10 minutes
    • repeat for another cycle and drying done by Dr. Hartings

PXRD

  • Set up PXRD of PVA+Clay films at different concentrations.
    • Tray 1 -Clay powder-control
    • Tray 2 -8ppm by Madeleine
    • Tray 3 -2ppm by
    • Tray 4 -PVA film-control
    • Tray 5 -80ppm by Eleni
    • Tray 6 -200ppm by my group
      • Instrument Settings
        • 2o start angle
        • 40o stop angle
        • 1o/second scan speed (this seems too fast, will check on this)
        • 0.5o sampling width

Calculations

  • Dilutions of MG solutions
    • 200ppm*0.2mL=8ppm*5mL(vial with PVA film)
    • 80ppm*0.5mL=8ppm*5mL(vial with no film)
    • 80ppm*0.2mL=8ppm*2mL(vial with PVA film)

Results

UV-Vis was performed on the solutions of MG started on September 12 and the results can be found in the graph and chart below.

The concentration of MG in the film was calculated in the following way:


(Initial ppm)(.010L) = (Initial mass of MG in 10 mL of solution, mg)


[(Solution Absorbance post-experiment)/(molar abosorptivity of Malachite Green according to calibration curve from September 3rd = 0.1193/ppm)]x(Volume = 0.01L) = (Final mass of MG in solution, mg)


[(Initial mass of MG in Solution)-(Final mass of MG in solution, mg)]/(initial mass of film) = (Concentration of MG in film, mgMG/gFilm)

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