User:Michael S. Bible/Notebook/571/2014/10/15: Difference between revisions
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*The instrument was set to an excitation wavelength of '''λ = 280 nm'''. | *The instrument was set to an excitation wavelength of '''λ = 280 nm'''. | ||
*Emission spectra were collected in the range of '''λ = 300-550 nm'''. | *Emission spectra were collected in the range of '''λ = 300-550 nm'''. | ||
*Spectra data were integrated using Riemann sums of base 0.5 nm. | |||
== | ====Fluorescence of CaCl<sub>2</sub>-Dialyzed Protein Data==== | ||
[[Image:Fluorescence_Responses_of_Calcium_binded_proteins.png]] | |||
In the image above, there is a clear and highly linear relationship between the peak integration and log[CaCl<sub>2</sub>] between 50 μM and 5 mM concentrations of CaCl<sub>2</sub>. Additional tests should be done to determine if this is indeed the case and to see if the trend extends further in either direction. | |||
==Fluorescence Analysis of HCl-Dialyzed Lysozyme== | |||
*The Fluorescence Spectrometer was used to analyze the dialyzed Lysozyme and the solutions against which Lysozyme was dialyzed. | |||
*The instrument was set to an excitation wavelength of '''λ = 280 nm'''. | |||
*Emission spectra were collected in the range of '''λ = 300-550 nm'''. | |||
*Spectra data were integrated using Riemann sums of base 0.5 nm. | |||
====Fluorescence of HCl-Dialyzed Protein Data==== | |||
[[Image:Concentration_of_HCl_versus_peak_integration_of_post-dialysis_HCl_soaks.png]] | |||
In the image above, there is no clear relationship between the concentration of the HCl soak initially and its post-dialysis peak integration. | |||
[[Image:Concentration_of_HCl_versus_peak_integration_of_post-dialysis_protein_solutions.png]] | |||
There appears to be a highly linear relationship between the peak integration values at HCl concentration of 250 μM, 125 μM, and 30 μM. More tests are necessary to see if this is indeed the case. | |||
==Data== | ==Data== |
Revision as of 11:17, 20 October 2014
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Fluorescence Analysis of CaCl2-Dialyzed Lysozyme
Fluorescence of CaCl2-Dialyzed Protein DataIn the image above, there is a clear and highly linear relationship between the peak integration and log[CaCl2] between 50 μM and 5 mM concentrations of CaCl2. Additional tests should be done to determine if this is indeed the case and to see if the trend extends further in either direction. Fluorescence Analysis of HCl-Dialyzed Lysozyme
Fluorescence of HCl-Dialyzed Protein DataIn the image above, there is no clear relationship between the concentration of the HCl soak initially and its post-dialysis peak integration. There appears to be a highly linear relationship between the peak integration values at HCl concentration of 250 μM, 125 μM, and 30 μM. More tests are necessary to see if this is indeed the case. Data
NotesThis area is for any observations or conclusions that you would like to note.
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