User:Michael S. Bible/Notebook/571/2014/10/15

Biomaterials Design Lab

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Fluorescence Analysis of CaCl2-Dialyzed Lysozyme The Fluorescence Spectrometer was used to analyze the dialyzed Lysozyme. The instrument was set to an excitation wavelength of λ = 280 nm. Emission spectra were collected in the range of λ = 300-550 nm. Spectra data were integrated using Riemann sums of base 0.5 nm. Fluorescence of CaCl2-Dialyzed Protein Data In the image above, there is a clear and highly linear relationship between the peak integration and log[CaCl2] between 50 μM and 5 mM concentrations of CaCl2. Additional tests should be done to determine if this is indeed the case and to see if the trend extends further in either direction. Fluorescence Analysis of HCl-Dialyzed Lysozyme The Fluorescence Spectrometer was used to analyze the dialyzed Lysozyme and the solutions against which Lysozyme was dialyzed. The instrument was set to an excitation wavelength of λ = 280 nm. Emission spectra were collected in the range of λ = 300-550 nm. Spectra data were integrated using Riemann sums of base 0.5 nm. Fluorescence of HCl-Dialyzed Protein Data In the image above, there is no clear relationship between the concentration of the HCl soak initially and its post-dialysis peak integration. There appears to be a highly linear relationship between the peak integration values at HCl concentration of 250 μM, 125 μM, and 30 μM. More tests are necessary to see if this is indeed the case. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.