User:Michael S. Bible/Notebook/571/2014/10/01: Difference between revisions
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Procedure taken from [[User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support/2014/10/01|Dr. Fox]] | Procedure taken from [[User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support/2014/10/01|Dr. Fox]] | ||
==Data== | ==Data== | ||
Revision as of 12:32, 19 October 2014
 Biomaterials Design Lab
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Analysis of Dialysis Begun YesterdayPost-dialysis solutions from all of the wells in the dialysis chamber were analyzed using UV-Vis using the following protocol:
Procedure taken from Dr. Fox
Data
Figure 1 shows the corrected UV-Vis Bradford Spectra for all Post-Dialysis Solutions.
Figure 2 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the non-protein side of the dialysis chamber. From the negative absorbance values, it is clear that there is no protein present. This is expected since the dialysis tubing used had a molecular weight cut-off of 3500 g/mol.
Figure 3 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the protein side of the dialysis chamber. It is clear from the spectra that there is still a lot of protein in each of the chambers, and the post-dialysis concentration of Lysozyme in solution almost perfectly matches the concentration of Lysozyme in the stock solution.
Figure 4 shows the calculations done to determine the concentration of Lysozyme in each solution. From these calculations, it would seem that the actual concentration of stock Lysozyme solution is .005 g/L rather than .125 g/L as believed.
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