User:Michael S. Bible/Notebook/571/2014/10/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 00:26, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
Analysis of Dialysis Begun YesterdayPost-dialysis solutions from all of the wells in the dialysis chamber were analyzed using UV-Vis using the following protocol:
Procedure taken from Dr. Fox DataFigure 1 shows the corrected UV-Vis Bradford Spectra for all Post-Dialysis Solutions. Figure 2 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the non-protein side of the dialysis chamber. From the negative absorbance values, it is clear that there is no protein present. This is expected since the dialysis tubing used had a molecular weight cut-off of 3500 g/mol. Figure 3 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the protein side of the dialysis chamber. It is clear from the spectra that there is still a lot of protein in each of the chambers, and the post-dialysis concentration of Lysozyme in solution almost perfectly matches the concentration of Lysozyme in the stock solution. Figure 4 shows the calculations done to determine the concentration of Lysozyme in each solution. From these calculations, it would seem that the actual concentration of stock Lysozyme solution is .28 g/L rather than .5 g/L as believed. This is because the stock solution is diluted by a factor of 50 when it is mixed with Bradford reagent and buffer.
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