User:Michael S. Bible/Notebook/571/2014/09/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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The following graphs were produced using UV-Vis data collected analysis of each of the solutions above. It was observed that at concentrations above 20 μg/mL the calibration curve is no longer linear, so the second graph only includes concentrations of lysozyme < 20 μg/mL. | |||
[[Image:Bradford_UV_VIS_Chart.png|500px]] | |||
[[Image:Bradford_Calibration_Curve_9-23.png|500px]] | |||
==Notes== | ==Notes== |
Latest revision as of 00:20, 27 September 2017
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Objective
DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
The following graphs were produced using UV-Vis data collected analysis of each of the solutions above. It was observed that at concentrations above 20 μg/mL the calibration curve is no longer linear, so the second graph only includes concentrations of lysozyme < 20 μg/mL. NotesBradford Assay is only effective from 1μg/mL to 10μg/mL
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