User:Michael S. Bible/Notebook/571/2014/09/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
==Creating Calibration Curves for NaCl Solution and Glycine Buffer Solution==
Learn how to maintain an OpenWetWare Notebook.
 
*In order to create pH and conductivity curves for our 50 mM NaCl solution and 50 mM Glycine buffer solution, we first made dilutions of our stock solutions.  We made our dilutions in 14 mL Falcon tubes using the volumes of stock solution and water found in the chart in the data section. 
*pH and conductivity readings for each dilution were then obtained using a pH and conductivity meter.  The readings and calibration curves constructed using that data can be found in the data section.


==Description==
==Description==
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==Data==
==Data==


[[Image:Glycine_Buffer_and_NaCl_solution_Dilutions.png|500px]]
[[Image:Glycine_Buffer_and_NaCl_solution_Dilutions.png|1000px]]


[[Image:Glycine_Buffer_Solution_Data.png|1000px]]
[[Image:Glycine_Buffer_Solution_Data.png|1000px]]


[[Image:Glycine_Buffer_Solution_Conductivity_Calibration_Curve.png|500px]]
[[Image:Glycine_Buffer_Solution_Conductivity_Calibration_Curve.png|1000px]]


[[Image:Glycine_Buffer_Solution_pH_Calibration_Curve.png|500px]]
[[Image:Glycine_Buffer_Solution_pH_Calibration_Curve.png|1000px]]


[[Image:NaCl_Solution_Data.png|1000px]]
[[Image:NaCl_Solution_Data.png|1000px]]


[[Image:NaCl_Solution_Conductivity_Calibration_Curve.png|500px]]
[[Image:NaCl_Solution_Conductivity_Calibration_Curve.png|1000px]]


[[Image:NaCl_Solution_pH_Calibration_Curve.png|500px]]
[[Image:NaCl_Solution_pH_Calibration_Curve.png|1000px]]


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
*Today we also removed the dialysis beakers (for the dialysis that was started [[User:Michael_S._Bible/Notebook/571/2014/09/16|yesterday]]) from the shaker and transferred the solutions inside and outside of the tubing in each to Falcon tubes for storage until next week.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]

Latest revision as of 00:18, 27 September 2017

Biomaterials Design Lab Main project page
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Creating Calibration Curves for NaCl Solution and Glycine Buffer Solution

  • In order to create pH and conductivity curves for our 50 mM NaCl solution and 50 mM Glycine buffer solution, we first made dilutions of our stock solutions. We made our dilutions in 14 mL Falcon tubes using the volumes of stock solution and water found in the chart in the data section.
  • pH and conductivity readings for each dilution were then obtained using a pH and conductivity meter. The readings and calibration curves constructed using that data can be found in the data section.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Notes

  • Today we also removed the dialysis beakers (for the dialysis that was started yesterday) from the shaker and transferred the solutions inside and outside of the tubing in each to Falcon tubes for storage until next week.




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