User:Michael S. Bible/Notebook/571/2014/09/16: Difference between revisions
From OpenWetWare
(→Data) |
|||
Line 33: | Line 33: | ||
***A 3500 g/mol MWCO tube in Glycine Buffer soak. | ***A 3500 g/mol MWCO tube in Glycine Buffer soak. | ||
== | ==Dialysis Protocol== | ||
* | #Cut about a 3" length of dialysis tubing | ||
#*25,000 MWCO tubing is stored in an azide solution to prevent mold (use care; NaN<sub>3</sub> is toxic) | |||
#*keep the 25,000 MWCO tubing wet to prevent pore shrinkage | |||
#wash cut tubing with DI water, both inside and out | |||
#*3,500 MWCO and 15,000 MWCO are dry and need to be wet prior to filling | |||
#*25,000 MWCO needs to be rinsed to remove the sodium azide | |||
#flatten tubing and remove as much residual water as possible with gloves fingers | |||
#clamp one end 1/4 - 1/2" from edge | |||
#open other end and transfer your '''measured''' protein solution inside | |||
#carefully flatten open end and clamp, making sure no liquid escapes | |||
#rinse with DI water, particularly the ends, which may have residual protein solution on it | |||
#place into a 150 mL beaker (or 250 mL or 400 mL) | |||
#measure out 100 - 200 mL of your dialyzing solution | |||
#label beaker, cover with parafilm, and leave on shaker on low speed to help prevent gradients during dialysis | |||
==Notes== | ==Notes== |
Revision as of 15:20, 17 September 2014
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Dialysis of Colloid SampleProtocols for today were adopted from Dr. Fox's Notebook. Description
Dialysis Protocol
NotesThis area is for any observations or conclusions that you would like to note.
|