User:Michael S. Bible/Notebook/571/2014/09/09: Difference between revisions
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==Fluorometry== | ==Fluorometry== | ||
Below is a graph of the data acquired from fluorometry on 81.2 nM L-Tryptophan. | Below is a graph of the data acquired from fluorometry on 81.2 nM L-Tryptophan. A wavelength of 275 nm was used to excite the solution. The small molarity is the result of a 2500:1 dilution of a 203 μM solution of L-Tryptophan. Tryptophan was the only one of our 5 amino acids that produced any significant results in UV-Vis and was, therefore, the only amino acid that was tested using Fluorometry. | ||
[[Image:Fluorometer_Readings_on_L-Tryptophan_chart.png]] | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 15:41, 18 September 2014
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ObjectiveToday we took UV/Vis spectra of the amino acid solutions made on 9/3. Description
UV-Vis Absorbance Curves for Amino AcidsFluorometryBelow is a graph of the data acquired from fluorometry on 81.2 nM L-Tryptophan. A wavelength of 275 nm was used to excite the solution. The small molarity is the result of a 2500:1 dilution of a 203 μM solution of L-Tryptophan. Tryptophan was the only one of our 5 amino acids that produced any significant results in UV-Vis and was, therefore, the only amino acid that was tested using Fluorometry.
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