User:Michael S. Bible/Notebook/571/2014/08/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 00:13, 27 September 2017
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Colloid v. Fiber Formation
Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein. Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.
Kinetics of AuNP FormationEnough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). The test tubes were covered with foil and placed in an 80°C hot water bath. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.
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