User:Michael F. Nagle/Notebook/Chem 571/2013/02/27

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(Procedure)
Line 9: Line 9:
*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.  
*Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.  
**The following concentrations were used.
**The following concentrations were used.
-
{| {{table}}
 
-
| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
 
-
| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
 
-
| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
 
-
| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
-
| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
 
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| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
 
-
|-
 
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| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
 
-
|-
 
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| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
 
-
|-
 
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| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
 
-
|-
 
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| 18.87||7.45856E-05||0.052994171||13407.40741||||||
 
-
|-
 
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| 25.64||8.67403E-05||0.03900156||11528.66242||||||
 
-
|-
 
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| 40||9.94475E-05||0.025||10055.55556||||||
 
-
|-
 
-
|
 
-
|}
 
-
 
-
*10mM Quercetin stock was prepared.
 
-
*Cuvettes with the following solutions were prepared and analyzed.
 
-
**Today, we only had time to complete the first two.
 
-
 
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
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|-
|-
| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
| 0.5||0.02||0.00333||200||0.6||40||1.78||0.6||65||13||3
 +
|-
 +
|
 +
|}
 +
 +
*10mM Quercetin stock was prepared.
 +
*Cuvettes with the following solutions were prepared and analyzed.
 +
**Today, we only had time to complete the first two.
 +
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration [Ado] (uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Velocity (nm/sec)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/[Ado] (1/uM)'''
 +
| align="center" style="background:#f0f0f0;"|'''1/velocity (sec/nm)'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
| align="center" style="background:#f0f0f0;"|'''(-1)/Km'''
 +
| align="center" style="background:#f0f0f0;"|'''-0.022385428'''
 +
|-
 +
| 10.52||3.70166E-05||0.095057034||27014.92537||||Km||44.67191792
 +
|-
 +
| 12.34||5.13812E-05||0.081037277||19462.36559||||1/Vmax||2662.131337
 +
|-
 +
| 14.93||6.0221E-05||0.066979236||16605.50459||||Vmax||0.000375639
 +
|-
 +
| 18.87||7.45856E-05||0.052994171||13407.40741||||||
 +
|-
 +
| 25.64||8.67403E-05||0.03900156||11528.66242||||||
 +
|-
 +
| 40||9.94475E-05||0.025||10055.55556||||||
|-
|-
|  
|  

Revision as of 16:18, 5 March 2013

Project name Main project page
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Procedure

  • Procedure from yesterday was continued with the same concentrations. Yesterday two trials were completed. The third was completed today.
    • The following concentrations were used.
[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Volume Inhibitor Stock solution added [Inhibitor Stock Solution] (uM) [Final Inhibitor] (uM) Total Volume (mL)
0.50.020.0033352.631578950.610.526315791.780.665133
0.50.020.0033361.728395060.612.345679011.780.665133
0.50.020.0033374.626865670.614.925373131.780.665133
0.50.020.0033394.339622640.618.867924531.780.665133
0.50.020.00333128.20512820.625.641025641.780.665133
0.50.020.003332000.6401.780.665133
  • 10mM Quercetin stock was prepared.
  • Cuvettes with the following solutions were prepared and analyzed.
    • Today, we only had time to complete the first two.


Concentration [Ado] (uM) Velocity (nm/sec) 1/[Ado] (1/uM) 1/velocity (sec/nm) ' (-1)/Km -0.022385428
10.523.70166E-050.09505703427014.92537Km44.67191792
12.345.13812E-050.08103727719462.365591/Vmax2662.131337
14.936.0221E-050.06697923616605.50459Vmax0.000375639
18.877.45856E-050.05299417113407.40741
25.648.67403E-050.0390015611528.66242
409.94475E-050.02510055.55556

Data

  • The following chart was prepared from the UV/Vis absorbance data for the assay solutions without Quercetin.

Image:3_5data.JPG

  • Data for the two Quercetin trials completed today will be uploaded next week, along with the rest, once they are complete.




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