User:Michael F. Nagle/Notebook/Chem 571/2013/02/12: Difference between revisions
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== | ==Objective== | ||
* | Repeat ADA Assay 2 times for additional data | ||
==Description== | |||
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer | |||
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted: | |||
**Dilution Tables are located below | |||
==Concentration Tables== | |||
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)''' | |||
|- | |||
| 5000||200||800||1000||1 | |||
|- | |||
| 5000||150||850||750||1 | |||
|- | |||
| 5000||100||900||500||1 | |||
|- | |||
| 5000||40||960||200||1 | |||
|- | |||
| 5000||16||984||80||1 | |||
|- | |||
| 5000||6.4||993.6||32||1 | |||
|} | |||
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)''' | |||
|- | |||
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||750||0.6||150||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||500||0.6||100||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||200||0.6||40||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||80||0.6||16||2.38||3 | |||
|- | |||
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3 | |||
|} | |||
==Data== | |||
Run#1 | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)''' | |||
|- | |||
| 200||0.000116022 | |||
|- | |||
| 150||0.000192818 | |||
|- | |||
| 100||0.000139227 | |||
|- | |||
| 40||5.55556E-06 | |||
|- | |||
| 16||6.68508E-05 | |||
|- | |||
| 6.4||0.000039 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)''' | |||
|- | |||
| 0.005||8619.047619 | |||
|- | |||
| 0.006666667||5186.246418 | |||
|- | |||
| 0.01||7182.539683 | |||
|- | |||
| 0.025||180000 | |||
|- | |||
| 0.0625||14958.67769 | |||
|- | |||
| 0.15625||25641.02564 | |||
|} | |||
Run#2 | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)''' | |||
|- | |||
| 200||0.00021989 | |||
|- | |||
| 150||0.000147514 | |||
|- | |||
| 100||0.000144751 | |||
|- | |||
| 40||0.00010884 | |||
|- | |||
| 16||8.12155E-05 | |||
|- | |||
| 6.4||3.8674E-05 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)''' | |||
|- | |||
| 0.005||4547.738693 | |||
|- | |||
| 0.006666667||6779.026217 | |||
|- | |||
| 0.01||6908.396947 | |||
|- | |||
| 0.025||9187.817259 | |||
|- | |||
| 0.0625||12312.92517 | |||
|- | |||
| 0.15625||25857.14286 | |||
|} | |||
Revision as of 18:27, 21 February 2013
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ObjectiveRepeat ADA Assay 2 times for additional data Description
Concentration TablesThis table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
DataRun#1
|