User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

Objective

• Prepare stock solutions of adenosine and phosphate buffer
• Prepare aliquots of ADA stock solution
  • Stocks will be used for ADA activity assay next week

Preparation of adenosine stock solution

• 10mL of 15mM solution of adenosine was prepared
  • .015M = x/.01
  • 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
  • 401mg adenosine was weighed and added to 10mL phosphate buffer.
• The 15mM solution was diluted to 5mM
  • .01L * .015M = .005M * x
  • 0.03L total volume for 5mM
  • 0.02L buffer was added to bring the solution to 30mL and 5mM

Preparation of phosphate buffer

• 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
• pH was brought to 7.4 using NaOH

Preparation of ADA aliquots

• Dhea Patel measured out 1.7g 0.5unit/mL ADA
• Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
• This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.