User:Michael F. Nagle/Notebook/Chem 571/2012/11/27: Difference between revisions
From OpenWetWare
(Autocreate 2012/11/27 Entry for User:Michael_F._Nagle/Notebook/Chem_571) |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objectives== | ||
* | *Complete ADA activity assay | ||
*Prepare solutions with post-dialysis ADA and Au at same mole ratios that were analyzed [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/20|11/20]] | |||
==Procedure== | |||
*The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/07|54.4μM]] was used to prepare solutions of 25-200 [Au/ADA] | |||
**Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200 | |||
**Solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours. | |||
**[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/11/27|Mary Mendoza]] completed the ADA activity assay but the spectra aren't online so I won't have the data on here until I hear back about it's location | |||
Revision as of 19:07, 7 December 2012
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Procedure
| |
