User:Michael F. Nagle/Notebook/Chem 571/2012/11/07 - Revision history

Michael F. Nagle: /* Data */

Michael F. Nagle — Fri, 07 Dec 2012 11:51:28 GMT

Data

←Older revision		Revision as of 11:51, 7 December 2012
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-	<br>Frac 2 was found to be 42µM, while 8 was 54.4µM and 9 was 65µM	+	<br>Frac 2 was found to be 42µM, while 8 was 54.4µM and 9 was 65µM.

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Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Fri, 07 Dec 2012 11:51:13 GMT

Bradford Assay

←Older revision		Revision as of 11:51, 7 December 2012
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	<Br>		<Br>
	*A UV/Vis calibration curve was obtained by analyzing the samples and plotting BSA concentration against absorbance at 595nm.		*A UV/Vis calibration curve was obtained by analyzing the samples and plotting BSA concentration against absorbance at 595nm.
-	*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06\|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.	+	*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06\|ADA purified yesterday]], labelled Frac 9, was analyzed along with Fracs 2 and 8 purified [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/26\|9/26]] via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.

	==Data==		==Data==

Michael F. Nagle: /* Data */

Michael F. Nagle — Fri, 07 Dec 2012 11:49:37 GMT

Data

←Older revision		Revision as of 11:49, 7 December 2012
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		+	<br>Frac 2 was found to be 42µM, while 8 was 54.4µM and 9 was 65µM

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Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Fri, 07 Dec 2012 11:48:36 GMT

Bradford Assay

←Older revision		Revision as of 11:48, 7 December 2012
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	==Bradford Assay==		==Bradford Assay==

-	*~~A standard curve for UV/Vis was obtained~~ with the following concentrations of BSA:	+	*Solutions were prepared with 715μL 1x Bio-Rad Bradford reagant and the following concentrations of BSA:

	{\| {{table}}		{\| {{table}}
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	\|}		\|}
	<Br>		<Br>
		+	*A UV/Vis calibration curve was obtained by analyzing the samples and plotting BSA concentration against absorbance at 595nm.
	*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06\|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.		*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06\|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
		+
		+	==Data==
		+	[[Image:Bradforassay.png]]
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Fraction #'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Absorbance'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[ADA] [mg/mL]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''molar absorptivity [mL/mg*cm-1]'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Molarity (µM)'''
		+	\|-
		+	\| 2\|\|0.93\|\|0.0177\|\|52.4\|\|42.0
		+	\|-
		+	\| 8\|\|0.757\|\|0.0115\|\|65.9\|\|54.4
		+	\|-
		+	\| 9\|\|0.819\|\|0.0137\|\|59.7\|\|65.0
		+	\|-
		+	\|
		+	\|}
		+
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	\|}		\|}

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Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Fri, 07 Dec 2012 10:24:41 GMT

Bradford Assay

←Older revision		Revision as of 10:24, 7 December 2012
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	<Br>		<Br>
-	*The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.	+	*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06\|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

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Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Fri, 07 Dec 2012 10:24:06 GMT

Bradford Assay

←Older revision		Revision as of 10:24, 7 December 2012
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	==Bradford Assay==		==Bradford Assay==

-	*A standard curve was obtained with the following concentrations of BSA:	+	*A standard curve for UV/Vis was obtained with the following concentrations of BSA:

	{\| {{table}}		{\| {{table}}

Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Wed, 07 Nov 2012 18:57:59 GMT

Bradford Assay

←Older revision		Revision as of 18:57, 7 November 2012
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	==Bradford Assay==		==Bradford Assay==

-	A standard curve was obtained with the following concentrations of BSA:	+	*A standard curve was obtained with the following concentrations of BSA:

	{\| {{table}}		{\| {{table}}

Michael F. Nagle at 18:53, 7 November 2012

Michael F. Nagle — Wed, 07 Nov 2012 18:53:15 GMT

←Older revision		Revision as of 18:53, 7 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
		+
		+	==Objective==
		+	*Determine concentration of ADA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23\|Oct. 23]] via Bradford Assay
	==Bradford Assay==		==Bradford Assay==

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	\| 30\|\|0\|\|1\|\|1.51E-05		\| 30\|\|0\|\|1\|\|1.51E-05
	\|-		\|-
-	\|
	\|}		\|}
	<Br>		<Br>
-	*The ADA ~~made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23\|Oct. 23]]~~ was analyzed.	+	*The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
-	*The ADA's peak was compared to ~~those~~ of BSA's ~~linear regression~~ in order to determine concentration.	+
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

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Michael F. Nagle: /* Bradford Assay */

Michael F. Nagle — Wed, 07 Nov 2012 18:51:08 GMT

Bradford Assay

←Older revision		Revision as of 18:51, 7 November 2012
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	A standard curve was obtained with the following concentrations of BSA:		A standard curve was obtained with the following concentrations of BSA:

-	[table]	+	{\| {{table}}
-	~~[tr]~~	+	\| align="center" style="background:#f0f0f0;"\|'''volume BSA (uL)'''
-	~~[th]~~volume BSA (uL)~~[/th]~~	+	\| align="center" style="background:#f0f0f0;"\|'''volume H2O (uL'''
-	~~[th]~~volume H2O (uL~~)[/th]~~	+	\| align="center" style="background:#f0f0f0;"\|'''[BSA] (mg/mL)'''
-	[th]~~concentration BSA~~ (mg/mL)~~[/th]~~	+	\| align="center" style="background:#f0f0f0;"\|'''[BSA] (M)'''
-	[th]~~concentration BSA~~ (~~molarity~~)~~[/th]~~	+	\|-
-	~~[/tr]~~	+	\| 0\|\|30\|\|0\|\|0.00E+00
-	~~[tr]~~	+	\|-
-	~~[td]~~0~~[/td]~~	+	\| 5\|\|25\|\|0.24\|\|3.61E-06
-	~~[td]~~30~~[/td]~~	+	\|-
-	~~[td]~~0~~[/td]~~	+	\| 10\|\|20\|\|0.15\|\|2.26E-06
-	~~[td]~~0.00E+00~~[/td]~~	+	\|-
-	~~[/tr]~~	+	\| 15\|\|15\|\|0.13\|\|2.01E-06
-	~~[tr]~~	+	\|-
-	~~[td]~~5~~[/td]~~	+	\| 20\|\|10\|\|0.15\|\|2.26E-06
-	~~[td]~~25~~[/td]~~	+	\|-
-	~~[td]~~.24~~[/td]~~	+	\| 25\|\|5\|\|0.24\|\|3.61E-06
-	~~[td]~~3.61E-06~~[/td]~~	+	\|-
-	~~[/tr]~~	+	\| 30\|\|0\|\|1\|\|1.51E-05
-	~~[tr]~~	+	\|-
-	~~[td]~~10~~[/td]~~	+	\|
-	~~[td]~~20~~[/td]~~	+	\|}
-	~~[td]~~.15~~[/td]~~	+
-	~~[td]~~2.26E-06~~[/td]~~	+
-	~~[/tr]~~	+
-	~~[tr]~~	+
-	~~[td]~~15~~[/td]~~	+
-	~~[td]~~15~~[/td]~~	+
-	~~[td]~~.13~~[/td]~~	+
-	~~[td]~~2.01E-06~~[/td]~~	+
-	~~[/tr]~~	+
-	~~[tr]~~	+
-	~~[td]~~20~~[/td]~~	+
-	~~[td]~~10~~[/td]~~	+
-	~~[td]~~.15~~[/td]~~	+
-	~~[td]~~2.26E-06~~[/td]~~	+
-	~~[/tr]~~	+
-	~~[tr]~~	+
-	~~[td]~~25~~[/td]~~	+
-	~~[td]~~5~~[/td]~~	+
-	~~[td]~~.24~~[/td]~~	+
-	~~[td]~~3.61E-06~~[/td]~~	+
-	~~[/tr]~~	+
-	~~[tr]~~	+
-	~~[td]~~30~~[/td]~~	+
-	~~[td]~~0~~[/td]~~	+
-	~~[td]~~1~~[/td]~~	+
-	~~[td]~~1.51E-05~~[/td]~~	+
-	~~[/tr]~~	+
-	~~[/table]~~	+
	<Br>		<Br>
	*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23\|Oct. 23]] was analyzed.		*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23\|Oct. 23]] was analyzed.

Michael F. Nagle: /* Entry title */

Michael F. Nagle — Wed, 07 Nov 2012 18:48:57 GMT

Entry title

←Older revision		Revision as of 18:48, 7 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
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-	==~~Entry title~~==	+	==Bradford Assay==
-	* Insert content here...	+

		+	A standard curve was obtained with the following concentrations of BSA:

		+	[table]
		+	[tr]
		+	[th]volume BSA (uL)[/th]
		+	[th]volume H2O (uL)[/th]
		+	[th]concentration BSA (mg/mL)[/th]
		+	[th]concentration BSA (molarity)[/th]
		+	[/tr]
		+	[tr]
		+	[td]0[/td]
		+	[td]30[/td]
		+	[td]0[/td]
		+	[td]0.00E+00[/td]
		+	[/tr]
		+	[tr]
		+	[td]5[/td]
		+	[td]25[/td]
		+	[td].24[/td]
		+	[td]3.61E-06[/td]
		+	[/tr]
		+	[tr]
		+	[td]10[/td]
		+	[td]20[/td]
		+	[td].15[/td]
		+	[td]2.26E-06[/td]
		+	[/tr]
		+	[tr]
		+	[td]15[/td]
		+	[td]15[/td]
		+	[td].13[/td]
		+	[td]2.01E-06[/td]
		+	[/tr]
		+	[tr]
		+	[td]20[/td]
		+	[td]10[/td]
		+	[td].15[/td]
		+	[td]2.26E-06[/td]
		+	[/tr]
		+	[tr]
		+	[td]25[/td]
		+	[td]5[/td]
		+	[td].24[/td]
		+	[td]3.61E-06[/td]
		+	[/tr]
		+	[tr]
		+	[td]30[/td]
		+	[td]0[/td]
		+	[td]1[/td]
		+	[td]1.51E-05[/td]
		+	[/tr]
		+	[/table]
		+	<Br>
		+	*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23\|Oct. 23]] was analyzed.
		+	*The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.
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	\|}		\|}

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