User:Michael F. Nagle/Notebook/Chem 571/2012/11/07: Difference between revisions
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== | ==Bradford Assay== | ||
A standard curve was obtained with the following concentrations of BSA: | |||
[table] | |||
[tr] | |||
[th]volume BSA (uL)[/th] | |||
[th]volume H2O (uL)[/th] | |||
[th]concentration BSA (mg/mL)[/th] | |||
[th]concentration BSA (molarity)[/th] | |||
[/tr] | |||
[tr] | |||
[td]0[/td] | |||
[td]30[/td] | |||
[td]0[/td] | |||
[td]0.00E+00[/td] | |||
[/tr] | |||
[tr] | |||
[td]5[/td] | |||
[td]25[/td] | |||
[td].24[/td] | |||
[td]3.61E-06[/td] | |||
[/tr] | |||
[tr] | |||
[td]10[/td] | |||
[td]20[/td] | |||
[td].15[/td] | |||
[td]2.26E-06[/td] | |||
[/tr] | |||
[tr] | |||
[td]15[/td] | |||
[td]15[/td] | |||
[td].13[/td] | |||
[td]2.01E-06[/td] | |||
[/tr] | |||
[tr] | |||
[td]20[/td] | |||
[td]10[/td] | |||
[td].15[/td] | |||
[td]2.26E-06[/td] | |||
[/tr] | |||
[tr] | |||
[td]25[/td] | |||
[td]5[/td] | |||
[td].24[/td] | |||
[td]3.61E-06[/td] | |||
[/tr] | |||
[tr] | |||
[td]30[/td] | |||
[td]0[/td] | |||
[td]1[/td] | |||
[td]1.51E-05[/td] | |||
[/tr] | |||
[/table] | |||
<Br> | |||
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed. | |||
*The ADA's peak was compared to those of BSA's linear regression in order to determine concentration. | |||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 11:48, 7 November 2012
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Bradford AssayA standard curve was obtained with the following concentrations of BSA: [table]
[tr]
[th]volume BSA (uL)[/th]
[th]volume H2O (uL)[/th]
[th]concentration BSA (mg/mL)[/th]
[th]concentration BSA (molarity)[/th]
[/tr]
[tr]
[td]0[/td]
[td]30[/td]
[td]0[/td]
[td]0.00E+00[/td]
[/tr]
[tr]
[td]5[/td]
[td]25[/td]
[td].24[/td]
[td]3.61E-06[/td]
[/tr]
[tr]
[td]10[/td]
[td]20[/td]
[td].15[/td]
[td]2.26E-06[/td]
[/tr]
[tr]
[td]15[/td]
[td]15[/td]
[td].13[/td]
[td]2.01E-06[/td]
[/tr]
[tr]
[td]20[/td]
[td]10[/td]
[td].15[/td]
[td]2.26E-06[/td]
[/tr]
[tr]
[td]25[/td]
[td]5[/td]
[td].24[/td]
[td]3.61E-06[/td]
[/tr]
[tr]
[td]30[/td]
[td]0[/td]
[td]1[/td]
[td]1.51E-05[/td]
[/tr]
[/table]
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