User:Michael F. Nagle/Notebook/Chem 571/2012/11/07: Difference between revisions
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==Objective== | |||
*Determine concentration of ADA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] via Bradford Assay | |||
==Bradford Assay== | ==Bradford Assay== | ||
*Solutions were prepared with 715μL 1x Bio-Rad Bradford reagant and the following concentrations of BSA: | |||
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| 30||0||1||1.51E-05 | | 30||0||1||1.51E-05 | ||
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<Br> | |||
*A UV/Vis calibration curve was obtained by analyzing the samples and plotting BSA concentration against absorbance at 595nm. | |||
*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06|ADA purified yesterday]], labelled Frac 9, was analyzed along with Fracs 2 and 8 purified [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/26|9/26]] via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration. | |||
==Data== | |||
[[Image:Bradforassay.png]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Fraction #''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''[ADA] [mg/mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''molar absorptivity [mL/mg*cm-1]''' | |||
| align="center" style="background:#f0f0f0;"|'''Molarity (µM)''' | |||
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| 2||0.93||0.0177||52.4||42.0 | |||
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| 8||0.757||0.0115||65.9||54.4 | |||
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| 9||0.819||0.0137||59.7||65.0 | |||
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|} | |} | ||
< | <br>Frac 2 was found to be 42µM, while 8 was 54.4µM and 9 was 65µM. | ||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 22:13, 26 September 2017
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Objective
Bradford Assay
Data
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