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 Project name
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Objective
- Determine concentration of ADA prepared Oct. 23 via Bradford Assay
Bradford Assay
- A standard curve for UV/Vis was obtained with the following concentrations of BSA:
| volume BSA (uL)
|
volume H2O (uL
|
[BSA] (mg/mL)
|
[BSA] (M)
|
| 0 |
30 |
0 |
0.00E+00
|
| 5 |
25 |
0.24 |
3.61E-06
|
| 10 |
20 |
0.15 |
2.26E-06
|
| 15 |
15 |
0.13 |
2.01E-06
|
| 20 |
10 |
0.15 |
2.26E-06
|
| 25 |
5 |
0.24 |
3.61E-06
|
| 30 |
0 |
1 |
1.51E-05
|
- The ADA purified yesterday was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
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