User:Michael F. Nagle/Notebook/Chem 571/2012/11/06

Objectives

• Transform Adenosine Deaminase in cells
• Purify protein via Fast Protein Liquid Chromatography (FPLC)

Procedure

• The prodedure for transformation was followed, just like on 10/24 but with the following modifications
  • 20μL DNA solution was added to 30μL solution with E. Coli cells.
  • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
  • Single 200μL plates were prepared. No 50μL plates were made.
  • Plates were incubated at 37^oC
• Adenosine Deaminase prepared Oct. 23 was purified via FPLC as was done 9/26
  • Pump flow was 5mL/minute