User:Michael F. Nagle/Notebook/Chem 571/2012/11/06: Difference between revisions
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== | ==Objectives== | ||
* | *Transform Adenosine Deaminase in cells | ||
*Purify protein via Fast Protein Liquid Chromatography (FPLC) | |||
==Procedure== | |||
*The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications | |||
**20μL DNA solution was added to 30μL solution with E. Coli cells. | |||
**250μL Lysogeny Broth (LB) was used instead of SOC medium. | |||
**Single 200μL plates were prepared. No 50μL plates were made. | |||
*Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC | |||
** Pump flow was 5mL/minute | |||
Revision as of 11:31, 7 November 2012
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