User:Michael F. Nagle/Notebook/Chem 571/2012/11/06

(Difference between revisions)

Revision as of 14:31, 7 November 2012

Project name

The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications
- 20μL DNA solution was added to 30μL solution with E. Coli cells.
- 250μL Lysogeny Broth (LB) was used instead of SOC medium.
- Single 200μL plates were prepared. No 50μL plates were made.
Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC
- Pump flow was 5mL/minute

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-==Entry title==
+==Objectives==
-* Insert content here...
+*Transform Adenosine Deaminase in cells
+*Purify protein via Fast Protein Liquid Chromatography (FPLC)
+==Procedure==
+*The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications
+**20μL DNA solution was added to 30μL solution with E. Coli cells.
+**250μL Lysogeny Broth (LB) was used instead of SOC medium.
+**Single 200μL plates were prepared. No 50μL plates were made.
+*Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC
+** Pump flow was 5mL/minute