User:Michael F. Nagle/Notebook/Chem 571/2012/11/06: Difference between revisions
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==Procedure== | ==Procedure== | ||
*The prodedure for [[AU_Biomaterials_Design_Lab:Protocols/ | *The prodedure for [[AU_Biomaterials_Design_Lab:Protocols/Transformation_Protocol|transformation]] was followed, just like on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|10/24]] but with the following modifications | ||
**20μL DNA solution was added to 30μL solution with E. Coli cells. | **20μL DNA solution was added to 30μL solution with E. Coli cells. | ||
**250μL Lysogeny Broth (LB) was used instead of SOC medium. | **250μL Lysogeny Broth (LB) was used instead of SOC medium. | ||
**Single 200μL plates were prepared. No 50μL plates were made. | **Single 200μL plates were prepared. No 50μL plates were made. | ||
*Adenosine Deaminase prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was purified via FPLC | **Plates were incubated at 37<sup>o</sup>C | ||
*Adenosine Deaminase prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was purified via FPLC [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/26|as was done 9/26]] | |||
** Pump flow was 5mL/minute | ** Pump flow was 5mL/minute | ||
Revision as of 04:33, 7 December 2012
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