User:Michael F. Nagle/Notebook/Chem 571/2012/10/31

(Difference between revisions)

Revision as of 06:25, 7 December 2012

Project name

Au/Lysozyme samples prepared last Wednesday were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
Puja Moody purified ADA from E. Coli grown last week

@@ Line 11: / Line 11: @@
 ==Procedure==
 *Au/Lysozyme samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24| last Wednesday]] were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
-*Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
+*Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
 *[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody]] purified ADA from E. Coli grown [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]]