User:Michael F. Nagle/Notebook/Chem 571/2012/10/31
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| - | == | + | ==Objectives== |
| - | * | + | *Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios. |
| + | *Purify ADA from E. Coli | ||
| + | ==Procedure== | ||
| + | *Au/Lysozyme samples prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|last Wednesday] were analyzed by UV/Vis using disposable plastic cuvettes. The isntrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme. | ||
| + | *Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually. | ||
| + | *[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody] purified ADA from E. Coli grown [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week] | ||
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Revision as of 15:12, 31 October 2012
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