User:Michael F. Nagle/Notebook/Chem 571/2012/10/31: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 13: Line 13:
*Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
*Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
*[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody]] purified ADA from E. Coli grown [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]]
*[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody]] purified ADA from E. Coli grown [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]]
 
==Data==
 
[[Image:Quartzlys.jpg]]
 
==Discussion==
*In the abs. vs mole ratio plot for the first round, done using plastic cuvettes, a jagged line is seen from 134-140. A significant change in backgrounds is seem on the wavelength vs. absorbance graph. Because of this, we analyzed these samples again using quartz cuvettes.
*Starting at 100, an increasing amount of AuNPs appear in solution. This peaks at 140 before decining to near-zero at 170. This indicates that the optimal mole ratio for AuNPs in solution is 140 and that they start to go into fibers at higher mole ratios.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 04:11, 7 December 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios.
  • Purify ADA from E. Coli

Procedure

  • Au/Lysozyme samples prepared last Wednesday were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
  • Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
  • Puja Moody purified ADA from E. Coli grown last week

Data

Discussion

  • In the abs. vs mole ratio plot for the first round, done using plastic cuvettes, a jagged line is seen from 134-140. A significant change in backgrounds is seem on the wavelength vs. absorbance graph. Because of this, we analyzed these samples again using quartz cuvettes.
  • Starting at 100, an increasing amount of AuNPs appear in solution. This peaks at 140 before decining to near-zero at 170. This indicates that the optimal mole ratio for AuNPs in solution is 140 and that they start to go into fibers at higher mole ratios.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/10/31&oldid=662778"