User:Michael F. Nagle/Notebook/Chem 571/2012/10/31

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==Procedure==
==Procedure==
*Au/Lysozyme samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24| last Wednesday]] were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
*Au/Lysozyme samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24| last Wednesday]] were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
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*Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
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*Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
*[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody]] purified ADA from E. Coli grown [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]]
*[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody]] purified ADA from E. Coli grown [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]]
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==Data==
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[[Image:Quartzlys.jpg]]
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==Discussion==
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*In the abs. vs mole ratio plot for the first round, done using plastic cuvettes, a jagged line is seen from 134-140. A significant change in backgrounds is seem on the wavelength vs. absorbance graph. Because of this, we analyzed these samples again using quartz cuvettes.
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*Starting at 100, an increasing amount of AuNPs appear in solution. This peaks at 140 before decining to near-zero at 170. This indicates that the optimal mole ratio for AuNPs in solution is 140 and that they start to go into fibers at higher mole ratios.
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Objectives

  • Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios.
  • Purify ADA from E. Coli

Procedure

  • Au/Lysozyme samples prepared last Wednesday were analyzed by UV/Vis using disposable plastic cuvettes. The instrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
  • Samples from 134-140 showed variation in the background. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
  • Puja Moody purified ADA from E. Coli grown last week

Data

Image:Quartzlys.jpg

Discussion

  • In the abs. vs mole ratio plot for the first round, done using plastic cuvettes, a jagged line is seen from 134-140. A significant change in backgrounds is seem on the wavelength vs. absorbance graph. Because of this, we analyzed these samples again using quartz cuvettes.
  • Starting at 100, an increasing amount of AuNPs appear in solution. This peaks at 140 before decining to near-zero at 170. This indicates that the optimal mole ratio for AuNPs in solution is 140 and that they start to go into fibers at higher mole ratios.


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