User:Michael F. Nagle/Notebook/Chem 571/2012/10/24: Difference between revisions
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# 5μL of the DNA solution prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]] was added to 40μL cells | |||
# The solution was placed on a heat block for 30 seconds and put on ice. | |||
# The solution was incubated at 37<sup>o</sup>C and 225rpm for 1 hour | |||
## 250μL Lysogeny Broth (LB) was added | |||
# The solution was incubated again with the same specifications | |||
# Solutions with Lysozyme and BSA prepared [[[[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]]|last week]] were analyzed via UV/Vis. There was no peak at 520nm, so it was determined that heating at 50°C was insufficient. The solutions went back in the oven for 4 hours at 80°C, which is the same temperature BSA was seen succesfully unfolding at. | |||
# Solutions with Au and BSA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]] were analyzed via UV/Vis. | |||
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Revision as of 12:12, 24 October 2012
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